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. 2002 Nov 13;19(2):95–103. doi: 10.1016/0165-2427(88)90001-3

Antiviral and Antigenic Properties of Recombinant Porcine Interferon Gamma

B Charley 1, K McCullough 2, S Martinod 2
PMCID: PMC7133658  PMID: 2847405

Abstract

Recombinant porcine interferon gamma (rPoIFNγ) induced a dose-dependent inhibition of the cytopathic effect produced by vesicular stomatitis virus (VSV) challenge of both homologous and heterologous (bovine) cell lines. In addition, an antiviral effect of rPoIFNγ was demonstrable against the coronavirus transmissible gastroenteritis virus (TGEV) infection of porcine epithelial cells and of pulmonary macrophages. A rabbit anti-PoIFNγ antiserum was prepared and shown to specifically neutralize the antiviral effects of natural and recombinant porcine IFNγ preparations. This antiserum could also neutralize recombinant bovine IFNγ but not recombinant human IFNγ. These results suggest antigenic homology of porcine and bovine IFNγ but antigenic differences between these molecules and human IFNγ.

References

  1. Babiuk L.A., Bielefeldt Ohmann H., Gifford G., Czarniecki C.W., Scialli V.T., Hamilton E.B. Effect of bovine α1 interferon on bovine herpes virus type 1 induced respiratory disease. J. Gen. Virol. 1985;66:2283–2394. doi: 10.1099/0022-1317-66-11-2383. [DOI] [PubMed] [Google Scholar]
  2. Bielefeldt Ohmann H., Babiuk L.A. In vitro and systemic effects of recombinant bovine interferons on natural cell-mediated cytotoxicity in healthy and bovine herpes virus 1 infected cattle. J. Interferon Res. 1985;5:551–564. doi: 10.1089/jir.1985.5.551. [DOI] [PubMed] [Google Scholar]
  3. Bielefeldt Ohmann H.B., Babiuk L.A. Alteration of some leukocyte functions following in vivo and in vitro exposure to recombinant bovine alpha and gamma interferon. J. Interferon Res. 1986;6:123–136. doi: 10.1089/jir.1986.6.123. [DOI] [PubMed] [Google Scholar]
  4. Branca A.A., Baglioni C. Evidence that type I and II interferons have different receptors. Nature. 1981;294:768–770. doi: 10.1038/294768a0. [DOI] [PubMed] [Google Scholar]
  5. Capon D.J., Shepard M., Goeddel D.V. Two distinct families of human and bovine interferon α genes are coordinately expressed and encode functional polypeptides. Mol. Cell. Biol. 1985;5:768–779. doi: 10.1128/mcb.5.4.768. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ceretti D.M., McKereghan K., Larsen A., Cosman D., Gillis S., Baker P.E. Cloning, sequence and expression of bovine interferon γ. J. Immunol. 1986;136:4561–4564. [PubMed] [Google Scholar]
  7. Charley B., Fradelizi D. Differential effects of human and porcine interleukin 2 on natural killing (NK) activity of newborn piglets and adult pig lymphocytes. Ann. Rech. Vet. 1987;18:227–232. [PubMed] [Google Scholar]
  8. Charley B., Petit E., Leclerc C. Effects of intravenous injection of BCG or Freund adjuvant on swine alveolar macrophages. Vet. Immunol. Immunopathol. 1983;4:459–467. doi: 10.1016/0165-2427(83)90006-5. [DOI] [PubMed] [Google Scholar]
  9. Charley B., Petit E., Leclerc C., Stefanos S. Production of porcine interleukin 2 and its biological and antigenic relationships with human interleukin 2. Immunol. Lett. 1985;10:121–126. doi: 10.1016/0165-2478(85)90064-1. [DOI] [PubMed] [Google Scholar]
  10. Czarniecki C.W., Hamilton E.B., Fennie C.W., Wolf R.L. In vitro biological activities of Escherichia coli derived bovine interferon α, β and γ. J. Interferon Res. 1986;6:29–37. doi: 10.1089/jir.1986.6.29. [DOI] [PubMed] [Google Scholar]
  11. Haelterman E.D. On the pathogenesis of transmissible gastroenteritis of swine. J. Am. Vet. Med. Assoc. 1972;160:534–540. [PubMed] [Google Scholar]
  12. La Bonnardière C., Laude H. High interferon titer in newborn pig intestine during experimentally induced viral enteritis. Infect. Immun. 1981;32:28–31. doi: 10.1128/iai.32.1.28-31.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. La Bonnardière C., Laude H., Berg K. Biological and antigenic relationships between virus induced porcine and human interferons. Ann. Inst. Pasteur/Virol. 1986;137E:171–180. doi: 10.1016/S0769-2617(86)80202-7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Laude H. Thermal inactivation studies of a coronavirus, transmissible gastroenteritis virus. J. Gen. Virol. 1981;56:235–240. doi: 10.1099/0022-1317-56-2-235. [DOI] [PubMed] [Google Scholar]
  15. Laude H., Charley B., Gelfi J. Replication of enteropathogenic coronavirus TGEV in swine alveolar macrophages. J. Gen. Virol. 1984;65:327–332. doi: 10.1099/0022-1317-65-2-327. [DOI] [PubMed] [Google Scholar]
  16. Lefevre F., La Bonnardière C. Molecular cloning and sequencing of a gene encoding biologically active porcine α interferon. J. Interferon Res. 1986;6:349–360. doi: 10.1089/jir.1986.6.349. [DOI] [PubMed] [Google Scholar]
  17. Saklatvala J., Sarsfield S.J., Wood D.D. An antiserum to pig IL1 (Catabolin) reacts with the acidic but not the neutral form of human IL1. Br. J. Rheumatol. 1985;24(suppl. 1):68–71. [Google Scholar]
  18. Sauvagnac L. Contribution à l'étude de l'interféron porcin. Caractérisation de l'ARN messager de l'interféron gamma. Thèse Doct. Vet., Toulouse. 1987:150. [Google Scholar]
  19. Seow W.K., Thong Y.H. Augmentation of human polymorphonuclear leukocytes adherence by interferon. Int. Arch. Allergy Appl. Immunol. 1986;79:305–311. doi: 10.1159/000233991. [DOI] [PubMed] [Google Scholar]
  20. Sigel M.B., Sinha Y.N., Vanderlaan W.P. Production of antibodies by inoculation into lymph nodes. Methods Enzymol. 1983;93:3–12. doi: 10.1016/s0076-6879(83)93031-8. [DOI] [PubMed] [Google Scholar]
  21. Trinchieri G., Perussia B. Immune IFN: a pleiotropic lymphokine with multiple effect. Immunol. Today. 1985;6:131–135. doi: 10.1016/0167-5699(85)90080-5. [DOI] [PubMed] [Google Scholar]
  22. Weigent D.A., Langford M.P., Fleishmann W.R., Jr., Starton G.J. Potentiation of lymphocyte natural killing by mixture of alpha or beta interferon with recombinant gamma interferon. Infect. Immun. 1983;40:35–38. doi: 10.1128/iai.40.1.35-38.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Yilma T. Sensitivity of ovine choroid plexus cells to human and other animals IFNs. J. Gen. Virol. 1983;64:2013–2016. doi: 10.1099/0022-1317-64-9-2013. [DOI] [PubMed] [Google Scholar]

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