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. 1999 Mar 8;35(2):143–149. doi: 10.1016/S0167-7012(98)00110-9

Production and characterization of anti-peptide monoclonal antibodies with specificity for staphylococcal enterotoxins A and B

AR Bhatti a,*, VV Micusan b
PMCID: PMC7133826  PMID: 10192046

Abstract

A synthetic peptide containing selected epitopes from staphylococcal enterotoxin A (SEA) and enterotoxin B (SEB) was used to produce monoclonal antibodies (Mabs) to respective enterotoxins in a single fusion procedure. The peptide inhibited the reaction of polyclonal anti-SEA or anti-SEB antisera with their homologous enterotoxin, thus showing that the chosen epitopes are part of the antibody-inducing enterotoxin sequences. Two Mabs, Mab-A and Mab-B, reacted with both the peptide and with either SEA or SEB. Used in a double antibody sandwich ELISA, the Mabs were able to quantitate the native SEA or SEB toxins at nanogram levels.

Keywords: S. aureus, Enterotoxins, Monoclonal antibodies

Footnotes

In conducting the research described in this report, the investigators adhered to the “Guide to Care and Use of Experimental Animals” published by Canadian Council on Animal Care.

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