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. 2000 Feb 25;36(2):247–257. doi: 10.1016/0168-1702(95)00008-E

Sequence and in vitro expression of the phosphoprotein gene of avian pneumovirus☆

Roger Ling a,1, Philip J Davis b,2, Yu Qingzhong b,3, Carla M Wood a, Craig R Pringle a, David Cavanagh b, Andrew J Easton a,∗
PMCID: PMC7133856  PMID: 7653102

Abstract

The phosphoprotein (P) gene of two subgroup A strains of avian pneumovirus comprised 855 nucleotides containing only one substantial open reading frame encoding a protein of 278 amino acids, with a predicted Mr of 30,323. In vitro translation of P mRNA in a wheat germ system resulted in the synthesis of two polypeptides of Mr 35,000 Comparison of the deduced P protein sequence with that of the known mammalian pneumoviruses revealed overall amino acid identities ranging from 31 to 34.5%, suggesting a distant relationship. However, there was a much higher identity (63.2–68.4%) in a region of 57 residues, which included a heptad repeat sequence.

Keywords: Avian, Pneumovirus, Phosphoprotein, In vitro translation

Footnotes

☆

The sequence reported here has been deposited in the GenBank database under accession number U22110.

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