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. 2002 Nov 13;3(4):373–384. doi: 10.1016/0168-1702(85)90437-X

Multiple sites of recombination within the RNA genome of foot-and-mouth disease virus

Andrew MQ King 1,∗, David McCahon 1, Keith Saunders 1,∗∗, John WI Newman 1, William R Slade 1
PMCID: PMC7134178  PMID: 3000107

Abstract

Recombinant foot-and-mouth disease viruses were isolated from cells infected with a mixture of temperature-sensitive (ts) mutants belonging to different subtype strains. In order to select for recombination events in many different regions of the genome, crosses were performed between various pairs of mutants, with ts mutations in different regions of the genome. ts+ progeny were analysed by electrofocusing virus-induced proteins and RNase T1 fingerprinting of their RNA. All but 5 out of 43 independent isolates, from nine crosses, proved to have recombinant RNA genomes. Maps of these genomes, based on a knowledge of the locations of the unique oligonucleotides, were constructed. Most could be interpreted as being the products of single genetic cross-overs, although three recombinants were formed by two cross-overs each. Cross-overs in at least twelve distinct regions of the genome were identified. This evidence of a large number of recombination sites suggests that RNA recombination in picornaviruses is a general, as opposed to a site-specific, phenomenon.

Keywords: picornavirus, foot-and-mouth disease virus, recombination, RNA fingerprint

References

  1. Agol V.I., Grachev V.P., Drozdov S.G., Kolesnikova M.S., Kozlov V.G., Ralph N.M., Romonova L.I., Tolskaya E.A., Tyufanov A.V., Viktorova E.G. Construction and properties of intertypie poliovirus recombinants: first approximation mapping of the major determinants of neurovirulence. Virology. 1984;136:41–55. doi: 10.1016/0042-6822(84)90246-0. [DOI] [PubMed] [Google Scholar]
  2. Baric R.S., Stohlman S.A., Lai M.M.C. Characterization of replicative intermediate RNA of mouse hepatitis virus: presence of leader RNA sequences on nascent chains. J. Virol. 1983;48:633–640. doi: 10.1128/jvi.48.3.633-640.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cech T.R. RNA splicing: three themes with variations. Cell. 1983;34:713–716. doi: 10.1016/0092-8674(83)90527-5. [DOI] [PubMed] [Google Scholar]
  4. Cooper P.D. A genetic map of poliovirus temperature sensitive mutants. Virology. 1968;35:584–596. doi: 10.1016/0042-6822(68)90287-0. [DOI] [PubMed] [Google Scholar]
  5. Forss S., Strebel K.,.Beck E., Schaller H. Nucleotide sequence and genome organization of foot-and-mouth disease virus. Nucleic Acids Res. 1984;12:6587–6601. doi: 10.1093/nar/12.16.6587. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Harris T.J.R., Robson K.J.H., Brown F. A study of nucleotide sequence conservation between RNAs of two serotypes of foot-and-mouth disease virus. J. Gen. Virol. 1980;50:403–418. doi: 10.1099/0022-1317-50-2-403. [DOI] [PubMed] [Google Scholar]
  7. Hirst G.K. Vol. 27. 1962. Genetic recombination with Newcastle disease virus polioviruses and influenza; pp. 303–308. (Cold Spring Harbor Symp. Quant. Biol.). [DOI] [PubMed] [Google Scholar]
  8. Jennings P.A., Finch J.T., Winter G., Robertson J.S. Does the higher order structure of the influenza virus nucleoprotein guide sequence rearrangements in influenza viral RNA? Cell. 1983;34:619–627. doi: 10.1016/0092-8674(83)90394-x. [DOI] [PubMed] [Google Scholar]
  9. Kew O.M., Nottay B.K. Evolution of the oral polio vaccine strain in humans occurs by both mutation and intermolecular recombination. In: Chanock R., Lerner R., editors. Modern Approaches to Vaccines. Cold Spring Harbor Laboratory; Cold Spring Harbor, NY: 1984. pp. 357–362. [Google Scholar]
  10. King A.M.Q., Newman J.W.I. Temperature-sensitive mutants of foot-and-mouth disease virus with altered structural polypeptides. I. Identification by electrofocusing. J. Virol. 1980;34:59–66. doi: 10.1128/jvi.34.1.59-66.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. King A.M.Q., McCahon D., Slade W.R., Newnian J.W.I. Recombination in RNA. Cell. 1982;29:921–928. doi: 10.1016/0092-8674(82)90454-8. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lake J.R., Priston R.A.J., Slade W.R. A genetic recombination map of foot-and-mouth disease virus. J. Gen. Virol. 1975;27:355–367. doi: 10.1099/0022-1317-27-3-355. [DOI] [PubMed] [Google Scholar]
  13. La Torre J.L., Underwood B.O., Lebendiker M., Gorman B.M., Brown F. Application of RNase-T1 one- and two-dimensional analyses to the rapid identification of foot-and-mouth disease viruses. Infect. Imm. 1982;36:142–147. doi: 10.1128/iai.36.1.142-147.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Ledinko N. Genetic recombination with poliovirus type 1. Studies of crosses between a normal horse serum-resistant mutant and several guanidine-resistant mutants of the same strain. Virology. 1963;20:107–119. doi: 10.1016/0042-6822(63)90145-4. [DOI] [PubMed] [Google Scholar]
  15. Lehtovaara P., Soderlund H., Keranen S., Pettersson R.F., Kaariainen L. Vol. 78. 1981. 18S defective interfering RNA of Semliki Forest virus contains a triplicated linear repeat; pp. 5353–5357. (Proc. Natl. Acad. Sci. U.S.A.). [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lowe P.A., King A.M.Q., McCahon D., Brown F., Newman J.W.I. Vol. 78. 1981. Temperature-sensitive RNA polymerase mutants of a picornavirus; pp. 4448–4452. (Proc. Natl. Acad. Sci. U.S.A.). [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. McCahon D., King A.M.Q., Roe D.S., Slade W.R., Newman J.W.I, Cleary A.M. Isolation and biochemical characterization of intertypic recombinants of foot-and-mouth disease virus. Virus Res. 1985;3:87–100. doi: 10.1016/0168-1702(85)90044-9. [DOI] [PubMed] [Google Scholar]
  18. McCahon D., Slade W.R. A sensitive method for the detection and isolation of recombinants of foot-and-mouth disease virus. J. Gen. Virol. 1981;53:333–342. doi: 10.1099/0022-1317-53-2-333. [DOI] [PubMed] [Google Scholar]
  19. McCahon D., Slade W.R., Priston R.A.J., Lake J.R. An extended genetic recombination map of foot-and-mouth disease virus. J. Gen. Virol. 1977;35:555–565. doi: 10.1099/0022-1317-35-3-555. [DOI] [PubMed] [Google Scholar]
  20. Monroe S.S., Schlesinger S. Common and distinct regions of defective-interfering RNAs of Sindbis virus. J. Virol. 1984;49:865–872. doi: 10.1128/jvi.49.3.865-872.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. O'Hara P.J., Nichol S.T., Horodyski F.M., Holland J.J. Vesicular stomatitis virus defective interfering particles contain extensive genomic sequence rearrangements and base substitutions. Cell. 1984;36:915–924. doi: 10.1016/0092-8674(84)90041-2. [DOI] [PubMed] [Google Scholar]
  22. Pringle C.R. Evidence of genetic recombination in foot-and-mouth disease virus. Virology. 1965;25:48–54. doi: 10.1016/0042-6822(65)90250-3. [DOI] [PubMed] [Google Scholar]
  23. Rueckert R.R., Wimmer E. Systematic nomenclature of picornaviral proteins. J. Virol. 1984;50:957–959. doi: 10.1128/jvi.50.3.957-959.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Saunders K., King A.M.Q. Guanidine-resistant mutants of aphthovirus induce the synthesis of an altered nonstructural polypeptide, P34. J. Virol. 1982;42:389–394. doi: 10.1128/jvi.42.2.389-394.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Saunders K., King A.M.Q., McCahon D., Newman J.W.I., Slade W.R., Forss S. Recombination and oligonucleotide analysis of guanidine-resistant foot-and-mouth disease mutants. J. Virol. 1985 doi: 10.1128/jvi.56.3.921-929.1985. (in press). [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Stanway G., Cann A.J., Hauptmann R., Hughes P., Clarke L.D., Mountford R.C., Minor P.D., Schild G.C., Almond J.W. The nucleotide sequence of poliovirus type 3 leon 12 a1b: comparison with poliovirus type 1. Nucleic Acids Res. 1983;11:5629–5643. doi: 10.1093/nar/11.16.5629. [DOI] [PMC free article] [PubMed] [Google Scholar]

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