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. 2014 Nov 11;27(11):841–848. doi: 10.3967/bes2014.110

Detection of Human Bocavirus in Children with Acute Respiratory Tract Infections in Lanzhou and Nanjing, China

Jian Jun WU a,c,⁁, Yu JIN a,b,⁁, Na LIN c, Zhi Ping XIE c, Jie Mei YU c, Jin Song LI c, Chang Qing CAO a,c, Xin Hui YUAN a,c, Jin Rong SONG a,c, Jing ZHANG a,c, Yang ZHAO a,c, Xiao Qian GAO a,c, Zhao Jun DUAN c,*
PMCID: PMC7134634  PMID: 25374017

Abstract

Objective

The aim of this study was to explore the prevalent characteristics of HBoV1 and its co-infection.

Methods

PCR was used to detect HBoV1-DNA (HBoV1) and other viruses. A multivariate logistic regression model was used to explore possibility of co-detected for related viruses.

Results

The positivity rates in Nanjing and Lanzhou were 9.38% (74/789) and 11.62% (161/1386), respectively (P<0.05). The HBoV1 positive group was younger than negative group (P<0.05). Seasonal differences were noted, with a higher frequency of infection in December and July. HBoV1-positive children [72.34% (169/235)] were co-infected with other respiratory viruses. Multifactorial analysis showed no correlations between HBoV1 and the clinical classification, region, gender, age, or treatment as an outpatient or in a hospital. Correlations were identified between HBoV1 infections with ADV (OR=1.53, 95% CI 1.03-2.28), RSV (OR=0.71, 95% CI 0.52-0.98), and IFVA (OR=1.77, 95% CI 1.00-3.13).

Conclusions

Presence of HBoV1 in nasopharyngeal aspirates did not correlate with region or gender, although the prevalence of HBoV1 was higher in younger children. There were no correlations between HBoV1 and other variables, except for the season and ADV, RSV, or IFVA infections.

Key words: Human bocavirus, Child, Respiratory tract infections

Biographies

Biographical notes of the first authors: WU Jian Jun, male, born in 1972, associate professor, PhD, major in applied microbiology

JIN Yu, female, born in 1960, chief physician, professor, doctoral tutor, major in pediatric clinical virus and pediatric digestive disease research.

Footnotes

This work was partly supported by a ‘973’ National Key Basic Research Program of China(Grant No. 2007CB310500), the Key Program of Nanjing Medical Science and Technique Development Foundation(Grant No. ZKX09008), and Medical Science and Technique Development Foundation of Jiangsu Province Health Department(Grant No. H200949).

References

  • 1.Hadler SC, Castro KG, Dowdle W. Epidemic Intelligence Service investigations of respiratory illness, 1946-2005. Am J Epidemiol. 2011;174:S36–S46. doi: 10.1093/aje/kwr309. [DOI] [PubMed] [Google Scholar]
  • 2.Mathisen M, Basnet S, Sharma A. RNA viruses in young Nepalese children hospitalized with severe pneumonia. Pediatr Infect Dis J. 2011;30:1032–1036. doi: 10.1097/INF.0b013e31822f845f. [DOI] [PubMed] [Google Scholar]
  • 3.Van Den Hoogen BG, De Jong JC, Groen J. A newly discovered human pneumovirus isolated from young children with respiratory tract disease. Nat Med. 2001;7:719–724. doi: 10.1038/89098. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 4.Fouchier RA, Hartwig NG, Bestebroer TM. A previously undescribed coronavirus associated with respiratory disease in humans. Proc Natl Acad Sci U S A. 2004;101:6212–6216. doi: 10.1073/pnas.0400762101. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 5.Woo PC, Lau SK, Tsoi HW. Clinical and molecular epidemiological features of coronavirus HKU1-associated community-acquired pneumonia. J Infect Dis. 2005;192:1898–1907. doi: 10.1086/497151. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 6.Woo PC, Lau SK, Chu CM. Characterization and complete genome sequence of a novel coronavirus, coronavirus HKU1, from patients with pneumonia. J Virol. 2005;79:884–895. doi: 10.1128/JVI.79.2.884-895.2005. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 7.Allander T, Tammi MT, Eriksson M. Cloning of a human parvovirus by molecular screening of respiratory tract samples. Proc Natl Acad Sci U S A. 2005;102:12891–12896. doi: 10.1073/pnas.0504666102. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 8.Nascimento-Carvalho CM, Ribeiro CT, Cardoso MR. The role of respiratory viral infections among children hospitalized for community-acquired pneumonia in a developing country. Pediatr Infect Dis J. 2008;27:939–941. doi: 10.1097/INF.0b013e3181723751. [DOI] [PubMed] [Google Scholar]
  • 9.Cevey-Macherel M, Galetto-Lacour A, Gervaix A. Etiology of community-acquired pneumonia in hospitalized children based on WHO clinical guidelines. Eur J Pediatr. 2009;168:1429–1436. doi: 10.1007/s00431-009-0943-y. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 10.Eyigor H, Osma U, Eyigor M. Detection of human bocavirus in children with upper respiratory tract infection by polymerase chain reaction. Clin Lab. 2013;59:139–142. doi: 10.7754/clin.lab.2012.120229. [DOI] [PubMed] [Google Scholar]
  • 11.Abdel-Moneim a S, Kamel MM, Al-Ghamdi a S. Detection of bocavirus in children suffering from acute respiratory tract infections in Saudi Arabia. PLoS One. 2013;8:e55500. doi: 10.1371/journal.pone.0055500. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 12.Xu L, He X, Zhang DM. Surveillance and genome analysis of human bocavirus in patients with respiratory infection in Guangzhou, China. PLoS One. 2012;7:e44876. doi: 10.1371/journal.pone.0044876. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 13.Jartti T, Hedman K, Jartti L. Human bocavirus-the first 5 years. Rev Med Virol. 2012;22:46–64. doi: 10.1002/rmv.720. [DOI] [PubMed] [Google Scholar]
  • 14.Liu WK, Chen DH, Liu Q. Detection of human bocavirus from children and adults with acute respiratory tract illness in Guangzhou, southern China. BMC Infect Dis. 2011;11:345. doi: 10.1186/1471-2334-11-345. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 15.Windisch W, Schildgen V, Malecki M. Detection of HBoV DNA in idiopathic lung fibrosis, Cologne, Germany. J Clin Virol. 2013;58:325–327. doi: 10.1016/j.jcv.2013.05.020. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 16.Jula A, Waris M, Kantola K. Primary and secondary human bocavirus 1 infections in a family, Finland. Emerg Infect Dis. 2013;19:1328–1331. doi: 10.3201/eid.1908.130074. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 17.Edner N, Castillo-Rodas P, Falk L. Life-threatening respiratory tract disease with human bocavirus-1 infection in a 4-year-old child. J Clin Microbiol. 2012;50:531–532. doi: 10.1128/JCM.05706-11. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 18.Kapoor A, Slikas E, Simmonds P. A newly identified bocavirus species in human stool. J Infect Dis. 2009;199:196–200. doi: 10.1086/595831. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 19.Arthur JL, Higgins GD, Davidson GP. A novel bocavirus associated with acute gastroenteritis in Australian children. PLoS Pathog. 2009;5:e1000391. doi: 10.1371/journal.ppat.1000391. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 20.Kapoor A, Simmonds P, Slikas E. Human bocaviruses are highly diverse, dispersed, recombination prone, and prevalent in enteric infections. J Infect Dis. 2011;201:1633–1643. doi: 10.1086/652416. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 21.Who . Integrated management of childhood illness handbook. Report WHO/FCH/CAH/00.12. World Health Organization; Geneva, Switzerland: 2000. [Google Scholar]
  • 22.Bezerra PG, Britto MC, Correia JB. Viral and atypical bacterial detection in acute respiratory infection in children under five years. PLoS One. 2011;6:e18928. doi: 10.1371/journal.pone.0018928. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 23.Cheng WX, Jin Y, Duan ZJ. Human bocavirus in children hospitalized for acute gastroenteritis: a case-control study. Clin Infect Dis. 2008;47:161–167. doi: 10.1086/589244. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 24.Bellau-Pujol S, Vabret A, Legrand L. Development of three multiplex RT-PCR assays for the detection of 12 respiratory RNA viruses. J Virol Methods. 2005;126:53–63. doi: 10.1016/j.jviromet.2005.01.020. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 25.Bastien N, Anderson K, Hart L. Human coronavirus NL63 infection in Canada. J Infect Dis. 2005;191:503–506. doi: 10.1086/426869. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 26.Vabret A, Dina J, Gouarin S. Detection of the new human coronavirus HKU1: a report of 6 cases. Clin Infect Dis. 2006;42:634–639. doi: 10.1086/500136. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 27.Hierholzer JC, Halonen PE, Dahlen PO. Detection of adenovirus in clinical specimens by polymerase chain reaction and liquid-phase hybridization quantitated by time-resolved fluorometry. J Clin Microbiol. 1993;31:1886–1891. doi: 10.1128/jcm.31.7.1886-1891.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 28.Wang K, Wang W, Yan H. Correlation between bocavirus infection and humoral response, and co-infection with other respiratory viruses in children with acute respiratory infection. J Clin Virol. 2010;47:148–155. doi: 10.1016/j.jcv.2009.11.015. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 29.Martin ET, Fairchok MP, Kuypers J. Frequent and prolonged shedding of bocavirus in young children attending daycare. J Infect Dis. 2010;201:1625–1632. doi: 10.1086/652405. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 30.Santos N, Peret TC, Humphrey CD. Human bocavirus species 2 and 3 in Brazil. J Clin Virol. 2010;48:127–130. doi: 10.1016/j.jcv.2010.03.014. [DOI] [PubMed] [Google Scholar]
  • 31.Cheng W, Chen J, Xu Z. Phylogenetic and recombination analysis of human bocavirus 2. BMC Infect Dis. 2011;11:50. doi: 10.1186/1471-2334-11-50. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 32.Pozo F, Garcia-Garcia ML, Calvo C. High incidence of human bocavirus infection in children in Spain. J Clin Virol. 2007;40:224–228. doi: 10.1016/j.jcv.2007.08.010. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 33.Kantola K, Hedman L, Arthur J. Seroepidemiology of human bocaviruses 1-4. J Infect Dis. 2011;204:1403–1412. doi: 10.1093/infdis/jir525. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 34.Zappa A, Canuti M, Frati E. Co-circulation of genetically distinct human metapneumovirus and human bocavirus strains in young children with respiratory tract infections in Italy. J Med Virol. 2011;83:156–164. doi: 10.1002/jmv.21940. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 35.Al-Rousan HO, Meqdam MM, Alkhateeb A. Human bocavirus in Jordan: prevalence and clinical symptoms in hospitalised paediatric patients and molecular virus characterisation. Singapore Med J. 2011;52:365–369. [PubMed] [Google Scholar]
  • 36.Bastien N, Brandt K, Dust K. Human Bocavirus infection, Canada. Emerg Infect Dis. 2006;12:848–850. doi: 10.3201/eid1205.051424. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 37.Fry A M, Lu X, Chittaganpitch M. Human bocavirus: a novel parvovirus epidemiologically associated with pneumonia requiring hospitalization in Thailand. J Infect Dis. 2007;195:1038–1045. doi: 10.1086/512163. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 38.Zeng M, Wang XH, Yu H. [Clinical relevance of human bocavirus with acute respiratory tract infection and diarrhea in children: a prospective case-control study] Zhonghua Er Ke Za Zhi. 2010;48:580–584. [PubMed] [Google Scholar]
  • 39.Weissbrich B, Neske F, Schubert J. Frequent detection of bocavirus DNA in German children with respiratory tract infections. BMC Infect Dis. 2006;6:109. doi: 10.1186/1471-2334-6-109. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 40.Choi EH, Lee HJ, Kim SJ. The association of newly identified respiratory viruses with lower respiratory tract infections in Korean children, 2000-2005. Clin Infect Dis. 2006;43:585–592. doi: 10.1086/506350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 41.Zhao B, Yu X, Wang C. High human bocavirus viral load is associated with disease severity in children under five years of age. PLoS One. 2013;8:e62318. doi: 10.1371/journal.pone.0062318. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 42.Bharaj P, Sullender WM, Kabra SK. Human bocavirus infection in children with acute respiratory tract infection in India. J Med Virol. 2010;82:812–816. doi: 10.1002/jmv.21637. [DOI] [PubMed] [Google Scholar]
  • 43.Tran DN, Nguyen TQ, Nguyen TA. Human bocavirus in children with acute respiratory infections in Vietnam. J Med Viro. 2014;86:988–994. doi: 10.1002/jmv.23789. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 44.Ricour C, Goubau P. Human bocavirus, a newly discovered parvovirus of the respiratory tract. Acta Clin Belg. 2008;63:329–333. doi: 10.1179/acb.2008.064. [DOI] [PubMed] [Google Scholar]
  • 45.Zhang Y, Teng Q, Ren C. Complete genome sequence of a novel reassortant H11N2 avian influenza virus isolated from a live poultry market in eastern China. J Virol. 2012;86:12443. doi: 10.1128/JVI.02236-12. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 46.Gao W, Sun W, Qu B. Distinct regulation of host responses by ERK and JNK MAP kinases in swine macrophages infected with pandemic (H1N1) 2009 influenza virus. PLoS One. 2012;7:e30328. doi: 10.1371/journal.pone.0030328. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 47.Maggi F, Andreoli E, Pifferi M. Human bocavirus in Italian patients with respiratory diseases. J Clin Virol. 2007;38:321–325. doi: 10.1016/j.jcv.2007.01.008. [DOI] [PubMed] [Google Scholar]
  • 48.Renois F, Talmud D, Huguenin A. Rapid detection of respiratory tract viral infections and coinfections in patients with influenza-like illnesses by use of reverse transcription-PCR DNA microarray systems. J Clin Microbiol. 2010;48:3836–3842. doi: 10.1128/JCM.00733-10. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 49.Honkinen M, Lahti E, Osterback R. Viruses and bacteria in sputum samples of children with community-acquired pneumonia. Clin Microbiol Infect. 2012;18:300–307. doi: 10.1111/j.1469-0691.2011.03603.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 50.Lusebrink J, Schildgen V, Tillmann RL. Detection of head-to-tail DNA sequences of human bocavirus in clinical samples. PLoS One. 2011;6:e19457. doi: 10.1371/journal.pone.0019457. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 51.Zheng LS, Yuan XH, Xie ZP. Human bocavirus infection in young children with acute respiratory tract infection in Lanzhou, China. J Med Virol. 2010;82:282–288. doi: 10.1002/jmv.21689. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 52.Streiter M, Malecki M, Prokop A. Does human bocavirus infection depend on helper viruses? A challenging case report. Virol J. 2011;8:417. doi: 10.1186/1743-422X-8-417. [DOI] [PMC free article] [PubMed] [Google Scholar]

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