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. 2004 Feb 24;1(5):934–939. doi: 10.1016/0955-0674(89)90061-6

Cell-to-cell fusion

JM White 1, CP Blobel 1
PMCID: PMC7134679  PMID: 2697297

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References

  • 1.Stegmann T., Doms R.W., Heienius A. Protein-mediated membrane fusion. Annu Rev Biophys Biophys Chem. 1989;18:187–211. doi: 10.1146/annurev.bb.18.060189.001155. Of outstanding interest. [DOI] [PubMed] [Google Scholar]; An excellent review of the role of proteins in biological membrane fusion events. The review focuses on the influenza virus HA, but good discussions of fusion by other viral proteins and by amphiphilic peptides are also presented. Several proteins implicated in cellular fusion events are also described.
  • 2.Wakelam N.J. Myoblast fusionA mechanistic approach. Curr Top Membr Transp. 1989;32:87–112. Of outstanding interest. [Google Scholar]; A well balanced review discussing factors involved in myoblast fusion and membrane-associated changes accompanying myoblast fusion. Several possible mechanisms of myoblast fusion are discussed.
  • 3.Yanagimachi Y. Sperm egg fusion. Curr Top Membr Transp. 1988;32:3–13. Of outstanding interest. [Google Scholar]; A comprehensive review of sperm--M fusion in a variety of systems. Factors regulating fusion as well as the effects of numerous perturbants are summarized. A specific hypothesis regarding the role of fusogenic proteins is proposed.
  • 4.Pizzey J.A., Jones G.E., Waish F.S. Requirements for the Ca2+-independent component in the initial intercellular adhesion of C2 myoblasts. J Cell Biol. 1988;107:2307–2318. doi: 10.1083/jcb.107.6.2307. Of interest. [DOI] [PMC free article] [PubMed] [Google Scholar]; The authors have developed an elegant assay for monitoring the initial stages of adhesion between C2 myoblasts. As observed in the avian system, they find that intercellular adhesion involves both a Ca2+-dependent and a Ca2+-independent system. The Ca2+-independent system is sufficient for initial cell-cell adhesion and appears to be composed, at least in part, of glycoproteins. Somewhat surprisingly the RGDS tetrapeptide stimulates Ca2+-independent adhesion.
  • 5.Florini J.R., Magri K.A. Effects of growth factors on myogenic differentiation. Am J Physiol (Cell Pbysiol) 1989;256:C701–C711. doi: 10.1152/ajpcell.1989.256.4.C701. Of interest. [DOI] [PubMed] [Google Scholar]; An up-to-date review discussing the processes of myoblast commitment and terminal differentiation. Emphasis is on the role of growth factors in these events.
  • 6.Enwlstie A., Zalin R.J., Bevan S., Warner A.E. The control of chick myoblast fusion by ion channels operated by prostaglandins and acetylcholine. J Cell Biol. 1988;106:1693–1702. doi: 10.1083/jcb.106.5.1693. Of interest. [DOI] [PMC free article] [PubMed] [Google Scholar]; This and the accompanying paper (Entwistle et al, J Cell Biol 1988, 106:1703–1712) suggest that ion channels affected by prostaglandins and acetylcholine are critically involved in controlling myoblast fusion. The authors suggest that depolarization of the myoblast membrane leads to a rise in intracellular calcium which, in turn, initiates myoblast fusion.
  • 7.Sauro V.S., Brown G.A., Hamilton M.R., Strickiand C.K., Strickland K.P. Changes in phospholipid metabolism dependent on calcium-regulated myoblast fusion. Biochem Cell Biol. 1988;66:1110–1118. doi: 10.1139/o88-128. Of interest. [DOI] [PubMed] [Google Scholar]; L6 rat myoblasts triggered to fuse by return to calcium-containing medium show a relatively rapid decrease (to 50% in 60 min) in PI 4,5 bisphosphate.
  • 8.Zachowski A., Henry J.-P., Devaux P.F. Control of transmembrane lipid asymmetry in chrornaffrn granules by an ATPdependent protein. Nature. 1989;340:75–76. doi: 10.1038/340075a0. Of interest. [DOI] [PubMed] [Google Scholar]; Spin-labeled phospholipids were used to demonstrate an ATP-dependent mechanism for maintaining high concentrations of PS in the cytoplasmic (endoplasmic) leaflet of chromaffin granules. Based on its sensitivity to N-ethylmaleimide, the authors propose that the aminophospholipid translocase activity is provided by the granule ATPase II. Aminophospholipid asymmetry may be a determinant of chromaffin granule-fusion competence.
  • 9.Urushiham H., Habata Y., Yanagisawa K. A membrane prorein with possible relevance to sexual cell fusion in Dia tyosteLIum discoideum. Cell Defer Develop. 1988;25:81–88. doi: 10.1016/0922-3371(88)90001-9. Of interest. [DOI] [PubMed] [Google Scholar]; Immunoadsorption studies suggest that a protein in the 70 kD range is involved in a post-aggregation step required for cell fusion in Dictyactelium discoideum.
  • 10.Boldt J., Howe A.M., Prebie J. Enzymatic alteration of the ability of mouse egg plasma membrane to interact with sperm. Biol Reprod. 1988;39:19–27. doi: 10.1095/biolreprod39.1.19. Of interest. [DOI] [PubMed] [Google Scholar]; Treatment of zona-free mouse eggs with trypsin or chymotrypsin inhibits sperm binding and subsequent fusion.
  • 11.Roe J.I., Farach A.H., Strittmatier W.J., Lennarz W.J. Evidence for involvement of metalloendoproteases in a step in sea urchin gamete fusion. J Cell Biol. 1988;107:539–544. doi: 10.1083/jcb.107.2.539. Of outstanding interest. [DOI] [PMC free article] [PubMed] [Google Scholar]; A careful study showing that metalloendoprotease inhibitors prevent fusion between sea urchin sperm and eggs without inhibiting sperm-egg binding. A previous study showed the involvement of metalloendoproteases in the acrosome reaction (Farach et al, J Biol Chem 1987, 262:5483–5487).
  • 12.Diaz-Perez E., Thomas P., Meizel S. Evidence suggesting a role for sperm metalloendoprotease activity in penetration of zona-free hamster eggs by human sperm. J Exp Zool. 1988;248:213–221. doi: 10.1002/jez.1402480213. Of interest. [DOI] [PubMed] [Google Scholar]; Metalloendoprotease inhibitors were shown to inhibit (by about 50%) the number of sperm that fuse per egg. The authors suggest that during or as a result of the acrosome reaction, a metalloendoprotease modifies the sperm plasma membrane, somehow increasing its fusion competence.

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