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. 2007 Sep 22;143:231–240. doi: 10.1016/S0923-2516(06)80111-1

The pattern of proteins synthesized in the liver is profoundly modified upon infection of susceptible mice with mouse hepatitis virus 3

Importantes modifications des protéines synthétisées par le foie des souris sensibles à l'infection par le virus 3 de l'hépatite murine

MA Lucchiari (1),(2), CA Pereira (1), L Kuhn (3), I Lefkovits (3),(*)
PMCID: PMC7135047  PMID: 1329165

Abstract

Susceptible BALB/c mice, after experimental infection with mouse hepatitis virus 3 (MHV3), revealed virus titres in the liver that increased gradually to a peak of 8 × 105 PFU/g of tissue after 3 days' infection, when the mice died of acute hepatitis. BALB/c mice were infected with MHV3, subsequently labelled in vivo with 35S-methionine, and then the liver preparations from both infected and non-infected animals were subjected to two-dimensional gel electrophoresis. Comparisons of the patterns by computer image analysis revealed 17 gene products which increased, and 8 gene products which decreased, upon virus infection in their two-dimensional gel spot intensity. We conclude that during MHV3 infection of a susceptible strain of mice, a major modification in protein synthesis occurs. The pattern alterations were not related to the virus gene products but were mostly endogenous mouse proteins. Whether these proteins are a result of a defence attempt by the animal, or are dictated by the virus in order to prevent a protective response from happening, remains to be shown.

Key-words: Coronavirus, Mouse hepatitis, Protein; Synthesis, Liver, Virus 3, In vivo labelling, Defences

References

  1. Allen R.C., Bienvenu J., Laurent P., Suskind R.M. Walter de Gruyer; New York: 1982. Marker proteins in inflammation. [Google Scholar]
  2. Arnheiter H., Baechi T., Haller O. Adult mouse hepatocytes in primary monolayer culture express genetic resistance to mouse hepatitis virus type 3. J. Immunol. 1982;129:1275–1281. [PubMed] [Google Scholar]
  3. Bang F.B., Warwick A. Vol. 46. 1960. Mouse macrophages as host cells for the mouse hepatitis virus and the genetic basis of their susceptibility; pp. 1065–1075. (Proc. natl. Acad. Sci. (Wash.)). [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dupuy J.M., Dupuy C., Decarie D. Genetically determined resistance to mouse hepatitis virus type 3 is expressed in hematopoietic donor cells in radiation chimeras. J. Immunol. 1984;133:1609–1615. [PubMed] [Google Scholar]
  5. Eaton J.W., Brandt P., Mahoney J.R. Haptoglobin: a natural bacteriostat. Science. 1982;215:691–693. doi: 10.1126/science.7036344. [DOI] [PubMed] [Google Scholar]
  6. Kettman J.R., Kuhn L., Young P., Lefkovits I. Parameters of the labelling of mitogen-activated murine lymphocytes by 35S-methionine for two-dimensional gel electrophoresis. I. — Effect of culture conditions. J. Immunol. Methods. 1986;88:53–57. doi: 10.1016/0022-1759(86)90051-7. [DOI] [PubMed] [Google Scholar]
  7. Kuschner I. The acute phase reactants and the erythrocyte sedimentation rate. In: Kelley W.W., Harris E.D., Ruddy S., Sledge C.B., editors. Textbook of rheumatology. W.B. Sanders; Berlin: 1981. p. 669. [Google Scholar]
  8. Kuschner I., Volanakis J.E., Gewurz H. C-reactive protein and the plasma protein response to tissue injury. Ann. N.Y. Acad. Sci. 1982:389. [PubMed] [Google Scholar]
  9. Lefkovits I., Young P., Kuhn L., Kettman J., Gemmell A., Tollaksen S., Anderson L., Anderson N. Use of a large-scale two-dimensional ISODALT gel electrophoresis system in immunology. In: Lefkovits I., Pernis B., editors. Vol. 3. Academic Press; Philadelphia: 1985. p. 163. (Immunological methods). [Google Scholar]
  10. Lefkovits L., Kettman J.R., Coleclough C. A strategy for founding a global lymphocyte proteinpaedia and gene catalog. Immunol. Today. 1990;11:157–162. doi: 10.1016/0167-5699(90)90066-i. [DOI] [PubMed] [Google Scholar]
  11. Levy G.A., Leibowitz J.L., Edginton T.S. Induction of monocyte procoagulant activity by murine hepatitis virus type 3 (MHV3) parallels disease susceptibility in mice. J. exp. Med. 1981;154:1150–1157. doi: 10.1084/jem.154.4.1150. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lucchiari M.A., Pereira C.A. A major role of macrophage activation by interferon gamma during mouse hepatitis virus type 3 infection. — I. Genetically dependent resistance. Immunobiol. 1989;180:12–22. doi: 10.1016/S0171-2985(89)80026-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lucchiari M.A., Pereira C.A. A major role of macrophage activation by interferon gamma during mouse hepatitis virus type 3 infection. — II. Age-dependent resistance. Immunobiol. 1990;181:31–39. doi: 10.1016/S0171-2985(11)80163-4. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Martin J.P., Koehren F., Rannou J.J., Kirn A. Temperature sensitive mutants of mouse hepatitis virus type 3 (MHV3): isolation, biochemical and genetic characterization. Arch. Virol. 1988;100:147–160. doi: 10.1007/BF01487679. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. O'Farrell P.H. High resolution two-dimensional electrophoresis of proteins. J. biol. Chem. 1975;250:4007–4021. [PMC free article] [PubMed] [Google Scholar]
  16. Pereira C.A., Steffan A.M., Kirn A. Interactions between mouse hepatitis viruses and primary cultures of Kupffer and endothelial liver cells from resistant and susceptible inbred mouse strains. J. gen. Virol. 1984;65:1617–1620. doi: 10.1099/0022-1317-65-9-1617. [DOI] [PubMed] [Google Scholar]
  17. Pereira C.A., Mercier G., Oth D., Dupuy J.M. Induction of natural killer cells and interferon during mouse hepatitis virus infection of resistant and susceptible inbred mouse strains. Immunobiol. 1984;166:35–42. doi: 10.1016/S0171-2985(84)80141-2. [DOI] [PubMed] [Google Scholar]
  18. Pluschke G., Lefkovits L. Two-dimensional gel pattern of proteins synthesized de novo in lymphoid organs of the mouse. Clin. Chem. 1984;30:2042–2047. [PubMed] [Google Scholar]
  19. Pluschke G., Jenni L., van Alphen L., Lefkovits I. Complete two-dimensional gel electrophoresis pattern of de novo synthesized acute phase reactants. Clin. exp. Immunol. 1986;66:331–339. [PMC free article] [PubMed] [Google Scholar]
  20. Tollaksen S.L., Anderson W.L., Anderson N.O. 6th ed. Argonne National Laboratory; New York: 1981. Operation of the ISODALT system, report ANL-RIM-81-1. [Google Scholar]
  21. Virelizier J.L., Gresser I. Role of interferon in the pathogenesis of viral diseases of mice as demonstrated by the use of anti-interferon serum. — V. Protective role in mouse hepatitis type 3 infection of susceptible and resistant strains of mice. J. Immunol. 1978;120:1616–1619. [PubMed] [Google Scholar]
  22. Weinberg E.D. Iron withholding: a defense against infection and neoplasia. Physiol. Rev. 1984;64:65. doi: 10.1152/physrev.1984.64.1.65. [DOI] [PubMed] [Google Scholar]

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