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. 2007 May 18;145:297–302. doi: 10.1016/S0923-2516(07)80034-3

The virulence of mouse hepatitis virus 3, as evidenced by permissivity of cultured hepatic cells toward escape mutants

Corrélation entre le pouvoir pathogène de deux mutants d'échappement du virus de l'hépatite murine 3 et la permissivité des cellules hépatiques en culture primaire

JP Martin (1),(*), W Chen (1), F Koehren (1), CA Pereira (1),(2)
PMCID: PMC7135458  PMID: 7839007

Abstract

Deux mutants d'échappement à la neutralisation du MHV3 (MHV 51.6 et MHV Cl12) montrent, en comparaison au MHV3 sauvage (wt), un ralentissement de leur multiplication chez des souris sensibles BALB/c et une atténuation du pouvoir pathogène provoquant un retard considérable de la mortalité. Le MHV3 wt se réplique à des titres élevés dans toutes les cellules hépatiques en culture. Le MHV3 51.6, de virulence intermédiaire, se multiplie dans toutes les cellules hépatiques excepté dans les cellules endothéliales. Le mutant qui tue le plus tardivement les souris (MHV Cl12) se multiplie uniquement dans les cellules de Ito, les autres étant non permissives. Ces résultats montrent une corrélation directe entre la virulence du MHV3 et son aptitude à se répliquer de manière efficace dans les cellules hépatiques in vitro.

Key-words: Hepatitis, MHV3, Replication, Permissivity; Hepatic cells, Escape mutants, Virulence

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