Abstract
At a concentration of 2 μg/ml, neither amphotericin B nor deoxycholate had an inactivating effect upon transmissible gastroenteritis Coronavirus in-fectivity. However, amphothericin B stimulated plaque formatin in agarose and facilitated the entry of viral RNA into swine testis cells. The combination of amphotericin B + deoxycholate inactivated virus infectivity and induced a decrease in plaque diameter. Finally, in the presence of these agents, the production of infectious virus and interferon was unchanged.
Key-words: TGE virus, Coronavirus, Fongizone, Amphotericin B, Deoxycholate, Gastroenteritis; Swine, In vitro
Résumé
A la concentration de 2 μg/ml, l’amphotéricine B et le désoxycholate n’ont pas d’effet inactivant sur le pouvoir infectant du coronavirus de la gastroentérite transmissible. En revanche, l’amphotéricine B stimule la formation des plages sous agarose et facilite l’entrée de l’ARN viral dans les cellules ST. L’association amphotéricine B + désoxycholate inactive le pouvoir infectant du virus et induit une diminution du diamètre des plages. Enfin, en présence de ces agents la production de virus infectieux et d’interféron n’est pas modifiée.
Mots-clés: Virus TGE, Fongizone, Amphotéricine B, Désoxycholate, Coronavirus, Gastroentérite; Porc, In vitro
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