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. 2004 Dec 10;144(2):111–120. doi: 10.1016/0923-2494(93)80066-8

Enrichment of coronavirus-induced interferon-producing blood leukocytes increases the interferon yield per cell: A study with pig leukocytes

W Nowacki 1, B Charley 1,*
PMCID: PMC7135579  PMID: 8390709

Summary

Porcine peripheral blood mononuclear cells, which secrete IFNα in response to a coronavirus, transmissible gastroenteritis virus, were detected by a filter immunoplaque assay (ELISPOT). IFNα-producing cells (IPC), which are present at a low frequency in the blood, could be enriched up to 100-fold by sequential depletion of plastic-adherent cells and cell fractionation on metrizamide density gradients. IPC were present in the non-adherent low-density cell subpopulation. Cell selection experiments using antibody (Ab)-coated immunomagnetic beads revealed that porcine IPC could be positively selected by anti-CD4 or -SLA-class-II Ab, but not by anti-CD2 or -CD8 Ab. The estimated IFN yield per IPC was found to increase when IPC were assayed at higher concentrations. These data suggest that IPC represent a unique and distinct cell population in the blood, which could secrete higher amounts of IFN following its accumulation at a site of viral infection.

Keywords: Coronavirus, Leukocyte, Interferon alpha, Transmissible gastroenteritis virus, IPC, ELISPOT, Immunobeads, PBMC, mAb

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