Abstract
Endogenous interferon (IFN) made by the newborn piglet in response to enteric coronavirus TGEV (transmissible gastroenteritis virus) infection was identified as leukocytic IFN (IFN-α). Indeed, the antiviral activity found in the serum and in the urine of infected piglets displayed the same main biological and antigenic properties as the IFN induced in influenza-infected pig leukocytes. It is therefore concluded that most, if not all, circulating IFN activity must be derived from lymphoid cells. Moreover, it was shown that a high degree of antigenic homology exists between porcine and human IFN-α: antibodies to HuIFN-α could efficiently neutralize PorIFN-α, and they were used for its purification by immunoaffinity.
Key-words: Interferon, Transmissible gastroenteritis, Influenza, Lymphoid cells, Swine, Man, Homology
Résumé
L'interféron (IFN) endogène synthétisé par le porcelet nouveau-né en réponse à l'infection par le virus de la gastro-entérite transmissible, a été identifié comme étant du type leucocytaire (IFN-α). L'activité antivirale détectée dans le sérum et l'urine des porcelets infectés présente en effet les mêmes propriétés biologiques et antigéniques que celles de l'IFN porcin produit en culture de leucocytes infectés par le virus grippal. Une origine essentiellement lymphoïde de l'IFN circulant est donc proposée. De plus, ce travail a mis en évidence une forte homologie antigénique entre l'IFN-α humain et l'IFN-α porcin: des anticorps dirigés contre l'IFN-α humain neutralisent très significativement l'IFN-α porcin et peuvent être utilisés par sa purification par immunoaffinité.
Mots-clés: Interféron, Gastro-entérite transmissible, Grippe, Cellules lymphoïdes, Porc, Homme, Homologie
Footnotes
Part of the present work was presented at the EEC Seminar on Adjuvants, interferon and non-specific immunity, held in Venice (Italy) April 27–28, 1983.
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