Specification Table

Subject Area:	Biochemistry, Genetics, and Molecular Biology
More specific subject area:	CRISPR/Cas9 Genome Editing
Method name:	Electroporating Cas9 and sgRNA expression vector plasmids into wall-less Chlamydomonas reinhardtii using exponential pulses
Name and reference of original method:	The original method is described in: A. Greiner, S. Kelterborn, H. Evers, G. Kreimer, I. Sizova, P. Hegemann, Targeting of Photoreceptor Genes in Chlamydomonas reinhardtii via Zinc-finger Nucleases and CRISPR/Cas9, Plant Cell. 29 (2017) 2498–2518. doi:10.1105/tpc.17.00659.
Resource availability:	www.chlamycollection.org for plasmids and cell strains used in the protocol