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(A) monobody fragment is generated from fragments involved in binding (orange), where the original monobody undergoes loss-of-function mutations in residues to remove affinity to the target and loss-of-stability mutations to disrupt beta-sheet packing (Red). (B) The presence of ligand and fragment allow a fragment-exchange complex to form that brings a Forster Resonance Energy Transfer (FRET) pair together, providing biosensors for the presence of a ligand.
