Figure 2.

TRPC5+PLSCR1 stimulates phosphatidylserine (PS) externalization in HEK293 cells. (A–F) Representative images showing TRPC5-mCherry expression and PS externalization on the plasma membrane of HEK293 cell transfected with empty vector (A–C) or TRPC5-mCherry+PLSCR1 (D–F). The cells were treated with saline as a control (A, D), a hypotonic solution (B, E) or LaCl₃ (100 μmol/L; C and F). (G–H) Summary data showing the PS externalized cells in percentage of total cells (FITC-positive). G: data from A–C; H: data from FITC channel in D–F. PS externalization was detected as green fluorescence via the annexin V-FITC assay. TRPC5 is detected as red fluorescence. Values are shown as the mean ± SEM (n = 3); *p < 0.05 for Control vs. Hypotonic or LaCl₃ with a two-tailed unpaired Student’s t test.