Figure 6.

Activation of TRPC5 stimulates phosphatidylserine (PS) externalization in mouse cerebral neurons. (A–E) Representative images showing PS externalization (green fluorescence) as determined by an annexin V-FITC assay in neurons of cerebral slices obtained from wild-type (A) or TRPC5 knockout (TRPC5 KO) mice (C) or normal C57 mice (B, D, E). (B) in the absence of extracellular Ca²⁺ (Ca²⁺ free); (D) treated with IgG; (E) treated with a functional blocking antibody T5E3. (F, G) Summary of average fluorescence intensity (arbitrary units) of PS externalized neurons in cerebral slices obtained from wild-type (wild type, Ca²⁺ free) and TRPC5 KO mice (F) or normal C57 mice treated with IgG or T5E3 (G). In each group, cerebral slices were treated with hypotonic buffer, daidzein (100 μmol/L) or LaCl₃ (100 μmol/L) for 10 min to activate TRPC5, or treated with saline as control. Values represent the mean ± SEM (n = 4-6); *p < 0.05, vs. control with a two-tailed unpaired Student’s t test; ^#p < 0.05, wild type vs. Ca²⁺ free or TRPC5 KO, or IgG vs. T5E3 with two-way ANOVA followed by the Tukey’s post-hoc comparison test.