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. 2020 Mar 19;9(3):758. doi: 10.3390/cells9030758

Figure 3.

Figure 3

Effects of Arp4 KD on gene expression. (A) Expression levels of OCT4, SOX2, UTF1, GDF3, and LEFTY1 mRNAs in Arp4 KD cells (Arp4-1 siRNA) were quantified and are shown relative to their respective levels in the control cells expressing control siRNA; (B) The expression level of OCT4 in control non-treated NIH3T3 cells, Arp4 KD cells, cells ectopically expressing NLS-actin (NLS-actin), and Arp4 KD cells ectopically expressing NLS-actin (Arp4 KD+NLS-actin). Values shown are relative to their respective levels in control cells; (C) The SRF-RE reporter plasmid was introduced into NIH3T3 cells expressing control-siRNA (control) or Arp4-1 siRNAs (Arp4 KD), and SRF responsive luciferase activity in each sample was measured as described in the Materials and Methods section. The plot shows activity relative to that in the control cells, the value for which was assigned as 1.0; (D) Quantitative RT-PCR analysis of mRNAs of SRF-targeting genes IGF-1 and ACTA2 in control and Arp4 KD cells. The expression level of each gene was normalized with respect to that of the GAPDH gene. Data shown are mean ± S.D. (n ≥ 3).