Appendix 1—figure 2. Top: Plot of displacement from a single GFP-SNX1 endosome trajectory in an MRC-5 cell (blue).

Shaded areas show persistent ($0.55<H<1$ in green) and anti-persistent ($0<H<0.45$ in magenta) behaviour. Middle: A 15 point moving window DLFNN exponent estimate for the trajectory (black) with a line (dashed) marking diffusion $H=0.5$ and lines (dotted) marking the confidence bounds $H=0.55$ and 0.45. Bottom: Plot of instantaneous and moving (15 point) window velocity. Right: Plot of the trajectory of a GFP-SNX1 endosome in an MRC-5 cell with start and finish positions, and persistent (green) and anti-persistent (magenta) segments indicated.