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. 2020 Feb 13;39(15):3102–3113. doi: 10.1038/s41388-020-1186-7

Fig. 5. Galectin-9 polarizes macrophages toward a M2 phenotype.

Fig. 5

a Macrophages were cocultured (1:4 ratio) with AsPC-1 cells with recombinant human galectin-9 or control for 48 h and stained for HLA-DR (top), CD86 (bottom) and b CD206. c After coculture for 48 h, macrophages were separated from AsPC-1 cells incubated with galectin-9 or control and then added to CD3+ T cells (1:2 ratio) for 48 h. T cells were stained for CD4, CD8, TNF-α (top) and IFN-γ (bottom). Bar graph, mean ± SEM, unpaired t-test. *p < 0.05, **p < 0.01, ***p < 0.001.