FIG 2.

BZA induced LC3B-II protein and increased the formation of autophagosomes and autolysosomes in macrophages. (A) PMA-differentiated THP-1 macrophages were infected with H37Ra (MOI = 10:1) for 6, 12, and 24 h in the presence or absence of BZA (5 μM). The LC3B-II protein level was analyzed by Western blotting. (B) mRFP-GFP-LC3B reporter THP-1 cells were differentiated by PMA and infected with H37Ra (MOI = 10:1) with or without BZA (5 μM) for 12 h. Representative confocal microscopy images are shown (bars, 5 μm). (C and D) The autophagosome puncta (yellow) per cell (C) and the autolysosome puncta (red) per cell (D) were calculated. The data represent the means ± standard deviations (SD) for three independent experiments. Values that are significantly different by one-way ANOVA are indicated by asterisks as follows: *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.