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. 2020 Mar 6;11(3):168. doi: 10.3390/insects11030168

Figure 2.

Figure 2

Gel images showing species-specific SCAR PCR results. Fragments of COI regions of (A) C. atrata, (B) M. opalifera, (C) P. kaempferi, and (D) H. maculaticollis were produced using CA_F1/CA_R1-1, MO_F1-1/MO_R2, PK_F1-1/PK_R1, and HM_F1-1/HM_R1 primer pairs, respectively. Primer sequences listed in Table 2. Lanes 1 to 4 correspond to Samples CA-1 to CA-4, respectively, of C. atrata; Lanes 5 and 6 correspond to Samples MO-1 and MO-2, respectively, of M. opalifera; Lanes 7 and 8 correspond to Samples PK-1 and PK-2, respectively, of P. kaempferi; Lanes 9 to 11 correspond to Samples HM-1 to HM-3, respectively, of H. maculaticollis. Information of 11 samples provided in Table 1. Lane M represents 100 bp DNA ladder. Arrows indicate precise sizes of PCR products.