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. 2020 Mar 1;11(3):155. doi: 10.3390/insects11030155

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of CCAP injection on oocyte growth and 20E titers in hemolymph. (A) Effect of CCAP injection on oocyte size in adult females. Adult females were injected daily with 10 pmol CCAP (dissolved in 5 μL of PBS), from day 1 to day 15 of the adult stage. Controls were injected with 5 μL PBS. (B) Effect of CCAP injections on ecdysteroid concentrations in the hemolymph. A total amount of 10 p moles of CCAP (in 5 μL of PBS) was injected daily into the hemocoel of female adults from day 1 to day 15. Control insects were injected with 5 μL of PBS. Ecdysteroid level was measured by the enzyme immunoassay (EIA). Data are expressed as mean ± SEM (n = 25 individuals/condition). ***: represent significant difference (p < 0.001) as compared to the control group, using Tukey’s test.