Figure 3.

Ultra performance liquid chromatography (UPLC) chromatograms of methanol extract of A. helferiana and standard-mixture (compounds 1 and 2). A suitable UPLC condition was established and the UPLC chromatogram of methanol extract (5 mg/mL) of A. helferiana (A) was obtained. The standard compound 1 and 2 were mixed (1 mg/mL each) and the UPLC chromatogram was obtained under similar condition (B) maintaining same column condition and solvent system. The peaks in chromatogram of methanol extract were identified as compounds 1 and 2 peaks after comparing with the retention time and UV-spectrum with that of standard compound peaks in chromatogram B.