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. 2020 Mar 3;12(3):685. doi: 10.3390/nu12030685

Figure 5.

Figure 5

Phase 2: Large-artery elastin and collagen in SHR. (A) Representative cross-sections (20× objective) of aorta stained for elastin (black) and collagen (red); (B) Elastin content in aorta as total area; (C) Elastin content in aorta as % media area; (D) Collagen content in aorta as total area; (E) Collagen content in aorta as % media area; (F) Aortic collagen:elastin ratio. Aortic cross-sections were stained with an Elastin Stain Kit (Sigma); ImageJ was used to quantify elastin (area of blackness) and collagen (area of redness) content. Inset: aortic cross-sections (4× objective). Scale bar applies equally to all aorta images (bar = 50 µm). Data are expressed as mean ± SEM (n = 4–7/group). Different letters represent significant differences (p < 0.05). An absence of letters indicates no statistical differences. Abbreviations: SHR, spontaneously hypertensive rat.