Figure 8.

EPS treatment blocks in ovo teratoma formation by undifferentiated hiPSCs. (A) Teratomas generated from NOD/SCID mice and chick chorioallantoic membrane (CAM) were sectioned, stained with H–E, and observed three germ layers (EcD: ectoderm, MeD: mesoderm, EnD: endoderm). (B) On ED day 10, matrigel plugs containing EPS-treated or untreated hiPSCs were placed on CAM and then further grown in an egg incubator. After 8 days, teratomas were photographed, excised from CAM, and then weighed. Scale bar = 10 mm. Data are expressed as mean ± SD (n = 3 or 4). ** p < 0.01 vs. EPS-untreated control.