Skip to main content
PMC home page
Elsevier - PMC COVID-19 Collection logoLink to Elsevier - PMC COVID-19 Collection
. 2013 Sep 13;33(2):200–204. doi: 10.1016/S0254-6272(13)60125-9

Antiviral activity of Jinchai capsule against influenza virus

Juying Zhong a, Xiaolan Cui b, Yujing Shi b, Yingjie Gao b, Hongxin Cao c,*
PMCID: PMC7147227  PMID: 23789217

Abstract

Objective

To evaluate the effect on influenza virus of Jinchai, a capsule made of Traditional Chinese Medicine.

Methods

Madin-darby canine kidney (MDCK) cells were infected with the FM1 strain of influenza virus A (subtype H1N1) in vitro. They were used to explore how Jinchai affected cell adsorption, cell membrane fusion, transcription and replication of the influenza virus. Hemagglutinin (HA) protein, intracellular pH, and influenza virus protein acid (PA) polymerase subunit were detected with confocal microscopy and real-time fluorescent quantitative polymerase chain reaction.

Results

Jinchai significantly reduced the expression of HA and PA polymerase subunit mRNA in infected MDCK cells. Jinchai also significantly decreased intracellular pH in infected cells.

Conclusions

Jinchai had strong anti-influenza activity against the influenza virus. It weakened the ability of the influenza virus to adsorb to cell wall and fuse with cell membranes in the early infection stage, and inhibited the transcription and replication of the virus.

Key words: Antiviral agents, Influenza A virus, Jinchai capsule

Footnotes

Supported byNational Significant New Drugs Creation-research and Development of Jinchai Antivirus Capsule (No.2009zx09301-005)

References

  • 1.Li Y. Chinese Medicine against respiratory viral infection pharmacological research. Shanxi Zhong Yi. 2007;23(4):71–72. [Google Scholar]
  • 2.Zhou XH, Zhen YJ, Feng JK. Scutellaria research. Hebei Zhong Yi Yao Xue Bao. 2000;15(3):31–34. [Google Scholar]
  • 3.Wang SC, Dang JY, Jia XD. Antipyretic and antiviral effect of radix bupleuri and scutellaria. Zhong Cao Yao. 1997;29(1):27. [Google Scholar]
  • 4.Zeng QF. Determination of trace elements and amino acids in Traditional Chinese Medicine Codonopsis. Zhong Guo Yi Yao Dao Bao. 2010;7(19):65–66. [Google Scholar]
  • 5.Cao HX, Wang XJ, Yu YH. An Ti Wei serum for Chinese Medicine and medicinal chemistry of anti SARS virus experiment. Zhong Guo Zhong Yao Za Zhi. 2004;29(3):281–283. [Google Scholar]
  • 6.Cao HX, Cui XL, Zhao J. An effective antiviral capsules for the study of An Ti Wei. Zhong Guo Zhong Yi Ji Chu Yi Za Zhi. 2004;10(10):771–774. [Google Scholar]
  • 7.Zhong JY, Cao HX, Cui XL. Effect of Jinchai antiviral capsules on prevention and treatment of influenza A H1N1 in immunodeficiency mouse. Zhong Guo Shi Yan Fang Ji Xue Za Zhi. 2010;16(13):109–112. [Google Scholar]
  • 8.Zhong JY, Cao HX, Cui XL. Effect of Jinchai antiviral capsules on prevention and treatment of influenza A H1N1 in mouse. Shi Jie Zhong Xi Yi Jie He Za Zhi. 2010;5(4):297–299. [Google Scholar]
  • 9.Reuter JD, Myc A, Hayes MM. Inhibition of viral adhesion and infection by sialic-acid-conjugated dendritic polymers. Bioconjug Chem. 1999;10(2):271–278. doi: 10.1021/bc980099n. [DOI] [PubMed] [Google Scholar]
  • 10.Euserink M. Virologymapmaker for the world of influenza. Science. 2008;320(5874):310–311. doi: 10.1126/science.320.5874.310. [DOI] [PubMed] [Google Scholar]
  • 11.Krishnan MN, Ng A, Sukumaran B. RNA interference screen for human genes associated with West Nile virus infection. Nature. 2008;455:242–245. doi: 10.1038/nature07207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 12.Brett DL, Heinz-Jurgen T, Charles M. Ricc: the viruses and their replication. Lippincott-Raven publishers; Philadelphia: 2007. pp. 1104–1105. [Google Scholar]
  • 13.Bullough PA, Hugson EM, Skehel JJ. Structure of influenza haemagglutinin at the pH of membrane fusion. Nature. 1994;371(6492):37–43. doi: 10.1038/371037a0. [DOI] [PubMed] [Google Scholar]
  • 14.Li ML, Rao P, Krug RM. The active sites of the influenza cap-dependent endonuclease are on different polymerase subunits. EMBO J. 2001;8(20):2078–2086. doi: 10.1093/emboj/20.8.2078. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 15.Guilligay D, Tarendeau F, Resa-Infante P. The structural basis for cap binding by influenza virus polymerase subunit PB2. Nat Struct Mol Biol. 2008;15:500–506. doi: 10.1038/nsmb.1421. [DOI] [PubMed] [Google Scholar]
  • 16.Yoshimoto J, Kakui M, Iwasaki H. Identification of a novel HA conformational change inhibitor of human influenza virus. Arch Virol. 1999;144(5):865–878. doi: 10.1007/s007050050552. [DOI] [PubMed] [Google Scholar]
  • 17.Fodor E, Crow M, Mingay LJ. A single amino acid mutation in the PA subunit of the influenza virus RNA polymerase inhibits endonucleolytic cleavage of capped RNAs. Virol. 2002;76(18):8989–9001. doi: 10.1128/JVI.76.18.8989-9001.2002. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 18.Kawaguchi A, Naito T, Nagata K. Involvement of influenza virus PA subunit in assembly of functional RNA polymerase comp lexes. Virol. 2005;79:732–744. doi: 10.1128/JVI.79.2.732-744.2005. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 19.Sugiura A, Tobita K, Eduin D. Isolation and characterization of temperature sensitive mutants of influenza virus. Virology. 1972;10(4):639–947. doi: 10.1128/jvi.10.4.639-647.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 20.Jung TE, Brownlee GG. A new prooter-binding site in the PBI subunit of the influenza A vitus polymerase. Gen Virol. 2006;87(3):679–688. doi: 10.1099/vir.0.81453-0. [DOI] [PubMed] [Google Scholar]
  • 21.Kawaguchi A, Naito T, Nagata K. Involvement of influenza vitus PA subunit in assembly of functional RNA polymerase complexes. Virol. 2005;79(2):732–744. doi: 10.1128/JVI.79.2.732-744.2005. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 22.Huarte M, Falcon A, Nakaya Y. Threonine 157 of influenza virus PA subunit modulates RNA replication in infecetious vituses. Virol. 2003;77(10):6007–6013. doi: 10.1128/JVI.77.10.6007-6013.2003. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 23.Fodor E, Mingay LJ, Crow M, Deng T, Brownlee GG. A single amino acid mutation in the PA subunit of the influenza vitus RNA polymerase promotes the generation of defective interfering RNAs. Virol. 2003;77(8):5017–5020. doi: 10.1128/JVI.77.8.5017-5020.2003. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 24.Shen J, Kirk BD, Ma JP, Wang QH. Diversifying selective pressure on influenza B virus hemagglutinin. Med Virol. 2009;81(1):114–124. doi: 10.1002/jmv.21335. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 25.Zurcher T, de la Luna S, Sanz-Ezquerro JJ. Mutational analysis of the influenza vitus A/Victoria/3/75 PA protein:studies of interaction with PB1 protein and identification of a dominant negative mutant. J Gen Virol. 1996;77(8):1745–1749. doi: 10.1099/0022-1317-77-8-1745. [DOI] [PubMed] [Google Scholar]
  • 26.Hara K, Shiota M, Kido H. Influenza vitus RNA polymerase PA subunit is a novel serine protease with Ser624 at the active site. Genes Cells. 2001;6(2):87–97. doi: 10.1046/j.1365-2443.2001.00399.x. [DOI] [PubMed] [Google Scholar]
  • 27.Rodriguez A, Perez-Gonzalez A, Nieto A. Influenza virus infection causes specific degradation of the largest subunit of cellular RNA polymerase II. Virol. 2007;81(10):5315–5324. doi: 10.1128/JVI.02129-06. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 28.Ezquerro JJ, Zurcher T, de la Luna S. The amino-terminal one-third of the influenza vitus RNA protein is responsible for the induction of proteolysis. Virol. 1996;70(2):1905–1911. doi: 10.1128/jvi.70.3.1905-1911.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 29.He XJ, Zhou J, Liu YF. Crystal structure of the polymerase PAC-PB1N complex from an avian influenza H5N1 virus. Nature. 2008;vol 454:1123–1126. doi: 10.1038/nature07120. [DOI] [PubMed] [Google Scholar]
  • 30.Cao HX, Cui XL, Zhao J. The mechanism of Antivirus capsules for the treatment of viral pneumonia in mice. Zhong Guo Zhong Yao Za Zhi. 2005;30(13):1039–1041. [Google Scholar]
  • 31.Cui XL, Cao HX, Zhao J. An experimental study for viagra capsules antipyretic. Zhong Yi Yao Xue Bao. 2005;33(3):57–59. [Google Scholar]
  • 32.Xu H, Cao HX, Liu JX. An Wei capsules effect on the delayed-type hypersensitivity immune function. Zhong Yi Yao Xue Kan. 2005;23(6):995–996. [Google Scholar]
  • 33.Cao HX, Wen WL. Plague of SARS. Zhong Yi Gu Ji Chu Ban She; Beijing: 2008. pp. 389–396. [Google Scholar]

Articles from Journal of Traditional Chinese Medicine are provided here courtesy of Elsevier

RESOURCES