Figure 2.

Evaluation of the ability of CuO-SiO₂@ZrO₂ NSPs to generate oxygen, the expression of HIF-1α and ^99mTc-HL91 labeled reoxygenation imaging experiment. (A) Picture of dissolved oxygen indicators for qualitative determination of oxygen production capacity of bare PBS (pH=5.5), dH₂O, CuO-SiO₂@ZrO₂ NSPs+dH₂O, CuO-SiO₂@ZrO₂ NSPs+PBS, CuO-SiO₂@ZrO₂ NSPs+MW+dH₂O and CuO-SiO₂@ZrO₂ NSPs+MW+PBS. (B) The dissolved oxygen concentration of the solution was quantitatively determined by a microcomputer DO-BOD detector. (C) Immunofluorescence staining of nucleus and HIF-1α after treatment with the IQuCS@Zr-PEG NSPS ((DAPI (blue) and anti-HIF-1 aipha antibody (green)). (D) Quantitative analysis of the region of interest was performed using radioactive counting methods. (E₁-E₅) The small animal SPECT scanning detects the reoxygenation status of the different groups, T indicates tumor, B indicates bladder.