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. 2020 Feb 25;12(3):253. doi: 10.3390/v12030253

Figure 5.

Figure 5

LACV spread through a population of HaCaT cells is limited by type I and type III IFN responses. (A,B) HaCaT cells were mock-treated or treated for 16 h with the indicated concentrations of exogenous IFN-β or IFN-λ1 (A), or a combination of both cytokines (B) before infection with LACV at a MOI of 5. At 8 hpi, cells were analyzed by flow cytometry for LACV Gc expression. Data is shown as percentage of untreated infected control. (C) HaCaT cells were treated with DMSO as a control or 1 μM Ruxolitinib for 16 h, followed by LACV infection at a MOI of 0.5. At indicated times, cells were analyzed by flow cytometry for Gc expression. (D) HaCat cells were first infected at a MOI of 0.5 PFU/cell and were then treated with 3 μg of neutralizing antibodies against IFN-β, a combination of antibodies to Type III IFNs (IL29, IL28a, and Human IFN Lambda Receptor 1) or the corresponding isotype control antibodies. At 24 hpi, cells were analyzed by flow cytometry for LACV Gc expression. In panel D, gray bar represents the percentage of Gc-positive cells in untreated LACV-infected cells. Values are the mean of three replicates with error bars indicating standard deviation and ** indicating p-values of <0.01 and *** indicating p-values of < 0.001.