Veltsos et al. (GENETICS 212: 815–835), entitled “Early Sex-Chromosome Evolution in the Diploid Dioecious Plant Mercurialis annua,” reported that the genome size for Mercurialis annua L. was 330 Mb, based on an estimate using the default model fitting with genomescope. This was erroneously interpreted as similar to the previously measured 1C haploid genome size of 640 Mb (Obbard et al. 2006).
The authors have now fitted the k-mer distribution accounting for the expected coverage of a haploid genome of 10 (genomescope.R -i fastq_countsM1_31.histo -o M1_31_prior_tr -l 10 -p 2 -k 31 —transform), using the latest version of genomescope (https://github.com/tbenavi1/genomescope/tree/9eaab4b27dccad6c8bf4e3a5883271b943412ff2). This analysis resulted in a 1C genome size estimate of 639 Mb (Figure S3), which is consistent with the previously published results from flow cytometry (Obbard et al. 2006). It also has a better error profile and a more realistic heterozygosity estimate (0.53% vs. 2.75%). Accordingly, relevant statements in the article—in the second full paragraph on p 818 and in the third full paragraph on p 821—have been corrected. See also Figure 1.
Figure 1.
The corrected GenomeScope profile, based on the latest version genomescope (https://github.com/tbenavi1/genomescope/tree/9eaab4b27dccad6c8bf4e3a5883271b943412ff2).
The authors would like to thank Mathias Scharmann for pointing out the error and Kamil S. Jaron for help in correcting this analysis. The Acknowledgments in the article have been altered accordingly.
Literature Cited
- Obbard D. J., Pannell J., and Harris S. A., 2006. Mercurialis canariensis (Euphorbiaceae), a new endemic to the Canary Islands. Kew Bull. 61: 99–106. [Google Scholar]