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. 2020 Mar 3;26(17):3862–3869. doi: 10.1002/chem.201905338

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© 2020 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Pull‐down of endogenous proteins from HEK293 whole cell lysate: (A) Pull‐down of endogenous SIRT2 using 3B‐Kmyr. Top membrane shows samples before pull‐down. Bottom membrane shows samples after pull‐down, including dose‐dependent competition of the adduct formation. (B) Pull‐down of endogenous SIRT5 using 4B‐Kglut. Top membrane shows samples before pull‐down. Bottom membrane shows samples after pull‐down, including dose‐dependent competition of the adduct formation. *All blots are performed in duplicates and can be seen in full in Figures S25 and S26.