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. 2019 Oct 11;71(3):1037–1054. doi: 10.1002/hep.30882

Figure 2.

Figure 2

Steap3 deficiency alleviates liver damage and inflammatory responses during hepatic I/R injury. (A) Steap3 protein expression in the liver of WT and Steap3‐KO mice. GAPDH served as the loading control (n = 3/group). (B) Serum ALT/AST activities in WT and Steap3‐KO mice at 6 and 24 hours after hepatic I/R operation (n = 8/group). (C) Representative histological H&E‐stained images and statistics showing necrotic areas in liver tissue from WT and Steap3‐KO mice at 6 and 24 hours after hepatic I/R operation (n = 6/group). Scale bar, 100 μm. (D) Serum levels of inflammatory factors (Tnf‐α and Ccl2) in WT and Steap3‐KO mice at 6 and 24 hours after hepatic I/R operation (n = 6‐8/group). (E) mRNA levels of proinflammatory factors (Il6, Il1b, Ccl2, and Cxcl10) in the liver of WT and Steap3‐KO mice at 6 and 24 hours after hepatic I/R operation (n = 6/group). (F) Representative CD11b immunofluorescence staining in the liver lobes of WT and Steap3‐KO mice at 6 hours after hepatic I/R operation (n = 5/group). Scale bar, 50 μm. (G) Protein levels of NF‐κB signaling pathway molecules in the liver of WT and Steap3‐KO mice at 6 hours after hepatic I/R operation. GAPDH served as the loading control (n = 3/group). All data are presented as the mean ± SD. Levels of statistical significance are indicated as *P < 0.05, **P < 0.01. For statistical analysis, one‐way ANOVA with Bonferroni’s post hoc analysis or Tamhane’s T2 post hoc analysis and two‐tailed Student t test were used. Abbreviations: HPF, high‐power field; IκBα, inhibitory κBα; IKKβ, IκB kinase β; n.s., not statistically significant.