Fig 6. Activation of Rac–GTP in malignant pancreatic lesions from K-RasG12/Vav1 mice.
Rac1–GTP activation in the pancreata of control, Vav1, K-RasG12D, and K-RasG12D/Vav1 mice was analyzed. (A) Western blotting using anti–Rac1–GTP Abs, anti-Rac1, and anti-actin were used to evaluate Rac1–GTP levels in protein lysates from pancreatic tissues of control, Vav1, K-RasG12D, and K-RasG12D/Vav1 mice (two mice in each group) at 5 mo after transgene induction. The vertical black lines delineate sliced images that juxtapose lanes that were nonadjacent in the gel. (B) The levels of Rac1–GTP versus total Rac1 at the pancreata of control, Vav1, K-RasG12D, and K-RasG12D/Vav1 mice at 1, 2, 3.5, and 5 mo after initiation of transgene induction were calculated. Each Rac1–GTP/Rac1 was then divided by the control ratio. Band intensity of the Western blots was quantified using ImageJ 1.49V software. Number of mice in these experiments are outlined in Table S1. SEM are shown. (C) Immunofluorescence of Rac1–GTP of the pancreata from 2 control, 3 Vav1, 3 K-RasG12D, and 4 K-RasG12D/Vav1 mice, 3.5 mo post-transgene induction was performed. Representative pictures are depicted. Scale bar represents 25 μm. All sections were also stained with anti–Alexa Flour 594 dye for background analysis and found negative.
Source data are available for this figure.
