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. 2020 Mar 9;9(3):201. doi: 10.3390/pathogens9030201

Table 5.

PCR primers with sequence used in this study.

Gene Primer Sequence Amplicon Size (bp) Annealing Temperature Reference
invA-F CGGTGGTTTTAAGCGTACTCT T 796 58 [52]
invA-R CGAATATGCTCCACAAGGTTA
Flic-C-F CCCGCTTACAGGTGGACTAC 433 58 [53]
Flic-C-R AGCGGGTTT TCGGTGGTTGT
aadA1-F TATCAGAGGTAGTTG GCGTCAT 484 55 [57]
aadA1-R GTTCCATAGCGTTAAGGTTTCATT
blaTEM-1-F CATTTCCGTGTCGCCCTTAT 793 56 [58]
blaTEM-1-R TCCATAGTTGCCTGACTCCC
floR-F AACCCGCCCTCTGGATCAAGTCAA 548 62 [57]
floR-R CAAATCACGGGCCACGCTGTATC
tetA-F GGTTCACTCGAACGACGTCA 577 57 [59]
tetA-R CTGTCCGACAAGTTGCATGA
intl1 A-F GGCATCCAAGCAGCAAGC 2000 55 [60]
intl1 A-R: AAG CAG ACT TGA CCT GAT