FIG 1.

Differentially expressed miRNAs in blood-derived macrophages after L. pneumophila infection. BDMs from three different donors were infected with L. pneumophila at an MOI of 0.25 or left untreated as a control (ctr). Samples were taken 24 and 48 h postinfection. RNA was isolated and used for library preparation and enriched for small RNAs using a TruSeq small RNA kit. Sequencing was performed in a multiplexed run of 1 × 51 cycle +7 (index). (A) Heatmap of the miRNA log₂ fold change over the respective controls. The expression of distinct upregulated (B and C) and downregulated (D and E) miRNAs in BDMs and THP-1 cells after L. pneumophila infection was validated by qPCR using TaqMan assays and are displayed as log₂ fold changes. Boxes show the upper and lower quartiles with medians and whiskers indicating minimal and maximal values of five to nine independent biological replicates for dysregulated miRNAs. Paired t tests were performed. *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ .001; ****, P ≤ 0.0001 (compared to the corresponding control).