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Protein expression by Ad5-ASFV constructs. The expression and authenticity of the ASFV antigens encoded by the generated recombinant adenoviruses were evaluated by immunocytometric analysis of Ad5-ASFV-infected HEK-293A cells probed with anti-FLAG monoclonal antibody [data not shown] and then validated by using ASFV-specific convalescent serum. Uninfected HEK-293A cells served as negative controls. Protein expression by representative constructs is shown.
