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. 2020 Mar 17;160(1):52–63. doi: 10.1111/imm.13180

Figure 2.

Figure 2

Expression of melatonin receptor 1A (MT1) and melatonin receptor 1B (MT2) in lymph nodes of non‐pregnant and pregnant ewes. (a) Expression values of MT1 and MT2 mRNA in the lymph node. (b) Expression of MT1 and MT2 proteins in the lymph node. (c) Representative immunohistochemical localization of MT1 protein in the lymph node. The lymph node is divided into an outer cortex (CO) and an inner medulla (ME). Lymph enters the convex through the subcapsular sinus (SS) and trabeculae (TRs) around the lymphoid nodules (LN) and flows into the medulla through the lymph sinus (LS) around the medullary cord (MC). Note: HE = stained by haematoxylin and eosin; Ctl = negative control; DN16 = day 16 of the oestrous cycle; DP13 = day 13 of pregnancy; DP16 = day 16 of pregnancy; DP25 = day 25 of pregnancy. Scale bar: 20 µm. P < 0·05 indicates significant difference.