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. 2020 May;26(5):550–563. doi: 10.1261/rna.072835.119

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© 2020 Teefy et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 2. — Epithelial piRNAs map predominantly to TE transcripts. Hywi- and Hyli-bound piRNAs isolated from (A) wild-type (WT) Hydra and (B) epithelial Hydra were mapped to the transcriptome. The piRNA mapping density (mapped piRNA counts per kb per million counts) is shown on the y-axis of the box and whisker plots. The results for piRNA mapping are subdivided by: (i) Hywi- or Hyli-bound piRNAs, (ii) sense (S) or antisense (AS) orientation, and (iii) transcript class (see legend). In all cases, TEs (red) have a significantly higher mapping density than other transcript classes as determined using Tukey's range test (P < 0.001; Supplemental Analysis 1).