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. 2009 Dec 4;65(12):1292–1300. doi: 10.1107/S0907444909040074

Structure of the X (ADRP) domain of nsp3 from feline coronavirus

Justyna A Wojdyla 1,2,3,4, Ioannis Manolaridis 1,2,3,4, Eric J Snijder 1,2,3,4, Alexander E Gorbalenya 1,2,3,4, Bruno Coutard 1,2,3,4, Yvonne Piotrowski 1,2,3,4, Rolf Hilgenfeld 1,2,3,4, Paul A Tucker 1,2,3,4
PMCID: PMC7161637  PMID: 19966415

Abstract

The structure of the X (or ADRP) domain of a pathogenic variant of feline coronavirus (FCoV) has been determined in tetragonal and cubic crystal forms to 3.1 and 2.2 Å resolution, respectively. In the tetragonal crystal form, glycerol‐3‐phosphate was observed in the ADP‐ribose‐binding site. Both crystal forms contained large solvent channels and had a solvent content of higher than 70%. Only very weak binding of this domain to ADP‐ribose was detected in vitro. However, the structure with ADP‐ribose bound was determined in the cubic crystal form at 3.9 Å resolution. The structure of the FCoV X domain had the expected macro‐domain fold and is the first structure of this domain from a coronavirus belonging to subgroup 1a.

Keywords: feline coronavirus, X domains, ADRP domains, nonstructural protein 3


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Articles from Acta Crystallographica Section D: Biological Crystallography are provided here courtesy of Wiley

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