Figure 5.

(a) Predicted secondary structure of the S. enterica CheW and A. baumannii CheW-like proteins. The green boxes indicate the S. enterica CheW domains involved in the interaction with RecA. (b) Results of a co-immunoprecipitation assay between the A. baumannii RecA-FLAG and A1S_2813-6× His proteins. The supernatants were separated by SDS-PAGE and assessed by western blotting. The image is representative of three independent experiments using three different lysates for each protein. The presence (+) or absence (−) of the RecA-FLAG or A1S_2813-6× His proteins in the corresponding lysate mixture is indicated, as is that of the antibody-coated beads used in each mixture. The RecA-FLAG and A1S_2813-6× His bands detected on a western blot are shown. M: molecular mass marker.