Downstream Processing: From Egg to Cell Culture‐Derived Influenza Virus Particles

M W Wolff; U Reichl

doi:10.1002/ceat.200800118

. 2008 May 27;31(6):846–857. doi: 10.1002/ceat.200800118

Downstream Processing: From Egg to Cell Culture‐Derived Influenza Virus Particles

M W Wolff ¹, U Reichl ^1,²

PMCID: PMC7162065 PMID: 32313385

Abstract

The establishment of cell culture‐derived vaccine production requires the development of appropriate downstream processes. Until today, many of the downstream methods applied originate from egg‐derived production processes. These methods have often been slightly modified in order to account for the new demands. However, efforts are currently underway to optimize these processes focusing, for example, on ion exchange or affinity based membrane adsorption chromatography. This review covers the main aspects relevant for the downstream processing of egg and mammalian cell culture‐derived whole influenza viruses.

Keywords: Downstream Processing, Influenza Virus, Vaccine production

Efforts to control the annual spread of influenza virus have centered on prophylactic vaccinations. The majority of licensed vaccines for human use are still produced in eggs. An overview of unit operations used for the downstream processing of egg and cell culture‐derived influenza viruses is given, focusing on commonly applied chromatographic methods.

graphic file with name CEAT-31-846-g001.jpg

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Downstream Processing: From Egg to Cell Culture‐Derived Influenza Virus Particles

M W Wolff

U Reichl

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Downstream Processing: From Egg to Cell Culture‐Derived Influenza Virus Particles

M W Wolff

U Reichl

Abstract

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