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. 2020 Mar 26;10(11):4871–4884. doi: 10.7150/thno.43539

Figure 3.

Figure 3

CD8 and CD4 cell infiltration in 8 Gy-sEV-immunized mice. (A-C) The lymph nodes, spleen, and blood of BALB/c mice were double-stained using anti-mouse fluorescein isothiocyanate (FITC)-anti-mouse CD3, peridinin chlorophyll protein complex (PerCP)-anti-mouse CD8, or allophycocyanin (APC)-anti-mouse CD4, and analyzed using flow cytometry to detect CD4 and CD8. (D-F) 4T1 lung metastases, 4T1 primary tumors, and H22 primary tumors were harvested at the end of the experiment and stained with anti-mouse CD8 (imaged at 200× magnification). P-values were determined using a two-tailed Student's t-test (*P < 0.05, **P < 0.01, ***P < 0.001).