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. 2020 Mar 26;10(11):4822–4838. doi: 10.7150/thno.42387

Figure 3.

Figure 3

Improved mitochondrial biogenesis by TFAM overexpression alleviates NaF-induced neuronal death by enhancing mitochondrial function. (A, B) RT-qPCR (A) and immunoblot (B) analyses of TFAM in SH-SY5Y cells. GAPDH was used as the internal control. (C) RT-qPCR analyses of relative mtDNA contents in SH-SY5Y cells. Quantification represents the levels of ND4 normalized to nuclear gene GAPDH. (D, E) RT-qPCR (D) and immunoblot (E) analyses of CO1, CO2, CO3, ATP6, ATP8 in SH-SY5Y cells. (F, G) Representative flow plots of MMP levels (F) and mitoROS production (G) in SH-SY5Y cells measured by flow cytometry. (H) Levels of cell viability in SH-SY5Y cells using CCK-8 assay. SH-SY5Y cells were infected with adenovirus overexpressing TFAM and 24 h later were treated with 60 mg/L NaF for 24 h. Data information: Data are presented as mean ± SD. Data were cumulative of at least three independent experiments. * P <0.05 is considered significant compared with Vector group and #P < 0.05 is considered significant from Vector+NaF group by one-way ANOVA test.