Protein kinases PknA and PknB independently and coordinately regulate essential Mycobacterium tuberculosis physiologies and antimicrobial susceptibility

Jumei Zeng; John Platig; Tan-Yun Cheng; Saima Ahmed; Yara Skaf; Lakshmi-Prasad Potluri; Daniel Schwartz; Hanno Steen; D Branch Moody; Robert N Husson

doi:10.1371/journal.ppat.1008452

. 2020 Apr 7;16(4):e1008452. doi: 10.1371/journal.ppat.1008452

Protein kinases PknA and PknB independently and coordinately regulate essential Mycobacterium tuberculosis physiologies and antimicrobial susceptibility

Jumei Zeng ¹, John Platig ², Tan-Yun Cheng ³, Saima Ahmed ⁴, Yara Skaf ⁵, Lakshmi-Prasad Potluri ¹, Daniel Schwartz ⁵, Hanno Steen ⁴, D Branch Moody ³, Robert N Husson ^1,^*

Editor: Helena Ingrid Boshoff⁶

PMCID: PMC7164672 PMID: 32255801

Abstract

The Mycobacterium tuberculosis Ser/Thr protein kinases PknA and PknB are essential for growth and have been proposed as possible drug targets. We used a titratable conditional depletion system to investigate the functions of these kinases. Depletion of PknA or PknB or both kinases resulted in growth arrest, shortening of cells, and time-dependent loss of acid-fast staining with a concomitant decrease in mycolate synthesis and accumulation of trehalose monomycolate. Depletion of PknA and/or PknB resulted in markedly increased susceptibility to β-lactam antibiotics, and to the key tuberculosis drug rifampin. Phosphoproteomic analysis showed extensive changes in protein phosphorylation in response to PknA depletion and comparatively fewer changes with PknB depletion. These results identify candidate substrates of each kinase and suggest specific and coordinate roles for PknA and PknB in regulating multiple essential physiologies. These findings support these kinases as targets for new antituberculosis drugs and provide a valuable resource for targeted investigation of mechanisms by which protein phosphorylation regulates pathways required for growth and virulence in M. tuberculosis.

Author summary

Tuberculosis is a major global health threat, with approximately 10 million new cases per year and 1.5 million deaths. Although tuberculosis is treatable, cure requires prolonged antibiotic courses and strains that are resistant to current treatments are increasingly common. Understanding how Mycobacterium tuberculosis adapts to the human host during infection can lead to new approaches to treat tuberculosis. A major adaptive mechanism is reversible protein phosphorylation mediated by protein kinases, which detect signals in the extracellular environment and transmit this information to regulate M. tuberculosis physiology. In this study we analyzed two M. tuberculosis kinases, PknA and PknB, by comparing strains in which these kinases are present to strains in which one or both of these kinases has been depleted. We identified features that differ between the kinase replete and kinase depleted strains including bacterial growth and viability, cell shape, the bacterial cell envelope and gene regulation. We also identified proteins that show decreased phosphorylation in the kinase depleted strains, which can result from direct or indirect effects of kinase depletion. These findings provide insight into how these kinases are important for M. tuberculosis pathogenesis and indicate that these kinases may be valuable targets for development of new TB drugs.

Introduction

The World Health Organization estimates that in 2018 10 million people developed tuberculosis (TB) and 1.5 million died from the disease [1]. Although tuberculosis is curable with antibiotics, current lengthy treatment regimens make completion of therapy difficult. Moreover, incomplete adherence to these prolonged treatment courses favors the emergence of rifampin-resistant/multidrug-resistant TB. Treatment of these resistant strains requires even longer courses with more drugs that are often poorly tolerated, resulting in lower adherence and rates of cure [2].

In addition to TB disease, Mycobacterium tuberculosis can cause an asymptomatic latent infection that can last for many years [3]. M. tuberculosis encounters many distinct environments in the host during the course of asymptomatic infection and active disease. These include short and long-term intracellular niches within phagocytes, and extracellular sites in a broad range of tissues and in necrotic caseum. M. tuberculosis requires mechanisms to sense signals in these host environments to adaptively regulate its cell physiology. Bacterial signal transduction mechanisms are also required for manipulation of host defenses by M. tuberculosis, a hallmark of tuberculosis pathogenesis. Together, these responses to host environments allow bacterial persistence and replication during M. tuberculosis infection and disease.

Reversible protein phosphorylation is a broadly conserved mechanism of transmembrane signaling that regulates cell physiology in response to signals from the extracellular environment. In most well-studied bacteria, two component systems are the predominant form of phosphorylation-based signal transduction [4]. In other bacteria, including M. tuberculosis and other Actinobacteria, Ser/Thr and Tyr protein kinases also play a major role in transmembrane signal transduction [5–10]. Ser/Thr and Tyr phosphorylation is the dominant phosphorylation-based signal transduction mechanism in eukaryotes, and aberrant function of kinase pathways has been linked to human disease, including several forms of cancer. As a result, several Tyr and Ser/Thr kinases have been targeted by small molecule inhibitors, many of which are in clinical use as approved drugs [11]. The conserved domain structure and mechanism of action of eukaryotic and bacterial protein kinases provide a foundation for developing small molecule inhibitors of essential bacterial Ser/Thr kinases that could serve as leads for drug development [12]. The success of such an approach would depend on optimizing selectivity for bacterial versus human kinases; that over 50 drugs approved for clinical use selectively target specific eukaryotic kinases [11], suggests that the development of potent and selective inhibitors of M. tuberculosis essential kinases may be achievable.

M. tuberculosis has 11 Ser/Thr kinases, two of which PknA and PknB (which also phosphorylates Tyr), are essential for bacteria viability [13, 14]. In prior studies, we and others have investigated the function of these kinases using genetic and chemical biology approaches [6, 8, 10, 15–21]. These investigations provide evidence that these kinases regulate, via reversible phosphorylation of regulators and enzymes, many functions required for M. tuberculosis growth and viability, including peptidoglycan synthesis and turnover, cell division, lipid metabolism, translation and central carbon metabolism [10, 17, 19, 22–24]. Consistent with their being essential for in vitro growth, depletion of PknA or PknB has been shown to result in lack of replication and markedly decreased lung pathology in a mouse model of infection [15, 16].

Knowledge of the direct substrates of the M. tuberculosis Ser/Thr kinases is essential for understanding their function and several protein substrates have been proposed for PknA and/or PknB, supported by evidence of varying strength [17]. Despite extensive study, the overlapping and discrete regulatory roles of M. tuberculosis PknA and PknB are not well understood.

Here we used a genetic depletion system in M. tuberculosis to investigate these kinases individually and as a pair. We compared kinase replete versus kinase-depleted bacteria using a combination of microscopy and microbiological, biochemical and phosphoproteomic approaches. We identified effects of kinase depletion on growth, viability, cell morphology, acid-fast staining and antimicrobial susceptibility. These phenotypes, together with concomitant changes in mycolic acid metabolism and extensive differences in protein phosphorylation between kinase replete and depleted strains, identify multiple components of the mycobacterial cell envelope, as well as several other essential cell physiologies, as targets of phosphorylation-based regulation by M. tuberculosis PknA and PknB. Our findings indicate distinct but partially overlapping roles for these essential M. tuberculosis kinases, support their potential as targets for anti-tuberculosis drug development, and provide a new resource for investigation of mechanisms by which reversible protein phosphorylation regulates pathways that are essential for growth and virulence of M. tuberculosis.

Results

Conditional expression of pknA, pknB and pknA+pknB

The genes encoding PknA and PknB are co-expressed in an operon in the M. tuberculosis chromosome. We used a pristinamycin (ptc)-inducible expression system [25] to conditionally deplete both pknA and pknB together or pknB individually in M. tuberculosis (S1A Fig). To achieve depletion of pknA alone, we complemented the pknA+pknB depletion strain with a pknB allele regulated by an anhydrotetracycline (atc)-inducible promoter (S1A and S1B Fig). Though the ptc-inducible promoter has been used for regulated gene expression in mycobacteria [25], it has not been shown to be titratable at the single cell level. Because this can be critical in interpreting phenotypes, we measured fluorescence intensity of individual cells containing an mCherry reporter [26] expressed from the ptc-inducible promoter. We found that this promoter is titratable at the single cell level, with graded increased activity in individual cells in response to increased inducer concentrations, shown by increased fluorescence Intensity (Fig 1). In comparison, a nitrile-inducible promoter that we previously characterized [27] is titratable primarily at the population level, manifested by a greater proportion of cells with increased fluorescence of similar intensity in response to increased nitrile concentration.

Fig 1 — **1A)** Titration of pristinamycin induction of mCherry expression under control of the pptr promoter was analyzed in an attenuated strain of M. *tuberculosis* (Δ*leuD*, Δ*panCD* [55]). Cells were induced for 48 hours, followed by fluorescence intensity measurement by FACS analysis to show the distribution of fluorescence intensity of individual cells. **1B)** The same reporter under control of the nitrile inducible promoter, which is not titratable at the single cell level [27], was used as a control. Data are representative of biological triplicates.

Because both kinase overexpression and depletion can affect growth, we titrated the expression of pknA and pknB to identify ptc concentrations that achieve kinase mRNA and protein levels similar to those present in wild type M. tuberculosis (Fig 2A–2C). To be able to analyze a strain in which only pknA was depleted, we determined the concentration of atc that achieved pknB expression in the pknA+pknB depletion strain that is similar to pknB expression in wild type (Fig 2D).

Fig 2 — Cells were grown in the presence of the indicated concentrations of ptc or atc for four days, followed by total RNA and protein isolation as described in the methods. For each panel the graph shows mRNA relative quantification by RT-qPCR and the image below each graph shows protein relative quantification by Western blotting using polyclonal rabbit anti-PknA antibody or monoclonal mouse anti-PknB antibody. Equal amounts of total RNA were used in each lane for RT-qPCR and equal amounts of total proein were loaded into each well for Western blotting. **2A)** titration of *pknA* mRNA and PknA protein in the *pknA+pknB* depletion strain in response to 2-fold dilutions of ptc. **2B)** titration of *pknB* mRNA and PknB protein in the *pknA+pknB* depletion strain. The membrane from panel 2A was stripped and re-probed with anti-PknB antibody. **2C)** titration of *pknB* mRNA and PknB protein in the *pknB* depletion strain. **2D)** titration of *pknA* and *pknB* mRNA and PknA and PknB protein in the *pknA* depletion strain. For these experiments, concentration of ptc when present was 0.25 μg/ml and concentrations of anhydrotetracycline were 10, 20, 40, or 200 ng/mL. Note that that there is a non-specific band that is present immediately above the PknA-specific band in panels 2A and 2D.

Growth and cell morphology effects of kinase depletion

All three depletion strains failed to form colonies when plated onto agar lacking inducer, confirming that pknA and pknB are both individually essential for growth (S1C Fig) [15]. We also performed growth curves in liquid medium over a range of inducer concentrations. Consistent with previous results [15, 16], we observed time-dependent growth defects in liquid medium for each strain in the absence of inducer (Fig 3A–3C). Depletion of pknA and especially pknB individually had more severe growth phenotypes than did depletion of both pknA and pknB together. We also observed decreases in cell viability after 4 days of kinase depletion, with pknA and pknB single depletion strains both showing greater decreases in colony forming units over time compared to pknA+pknB depletion (Figs 3D and S2). The more severe phenotypes of the pknA and pknB single depletion strains strain suggest that absence of one kinase may result in dysregulation of the activity of the non-depleted kinase in a manner that interferes with growth. A previous report showed a more severe growth phenotype for a pknA+pknB depletion strain generated using the same single crossover approach that we have used. The reason for this difference is not known, but may reflect differences in growth conditions, inducer concentrations or treatment of the cultures prior to initiation of depletion.

Fig 3 — Cultures were pre-grown in 7H9-ADN-Tw with 0.25 μg/ml ptc, pelleted, washed and diluted to achieve an initial OD₆₀₀ of 0.01. **3A)** growth curve of *pknA+pknB* depletion mutant with titration of ptc induction. **3B)** growth curve of *pknB* depletion mutant with titration of ptc induction, **3C)** growth curve of *pknA* depletion mutant with 0.25 μg/ml ptc or without induction, plus titrated induction of *pknB* by atc. Data for 3a-3c are the average of three biological replicates and error bars represent +/- 1 SD. **3D)** All three mutants were grown in liquid culture with or without 0.25μg/ml ptc induction; the atc concentration for the *pknA* depletion mutant was 20ng/ml. Serial dilutions (10⁻¹ to 10⁻⁵) of all three mutants were spotted on 7h9 agar at day 3, day 4, day 5 and day 6. Data are representative of duplicate experiments.

The growth defects seen in the kinase depletion strains led us to examine the morphology of M. tuberculosis cells following kinase depletion. Both single depletion strains and the pknA+pknB depletion strain showed significant cell shortening in the absence of kinase induction (Fig 4A and 4B). Given the localization of both kinases to the cell poles and septum, this phenotype may result from dysregulated cell division and/or polar growth [6, 10, 21]. A potential contributor the growth and morphology effects is the decreased phosphorylation in the PknB depletion strain of MmpS3 (LamA), recently described as a divisome protein that inhibits growth at the new cell pole following cell division [28]. The maintenance of rod-shaped morphology of these cells contrasts with the unipolar ballooning of cells in which divIVA (wag31), which is required for peptidoglycan synthesis at sub-polar sites, is depleted [29, 30].

Fig 4 — **4A)** Phase contrast microscopy of cells from wild type, *pknB*, or *pknA*+*pknB* depletion strain in the presence (0.25 μg/ml) or absence of ptc. For *pknA* depletion cells were grown in 0.25 μg/ml of ptc or 20 ng/ml atc. Pictures were taken with a 100x objective. Size bar = 5μM. **4B)** Distribution of length measurements of cells of wild type, *pknA*, *pknB* and *pknA*+*pknB* depletion mutants obtained with ImageJ software. More than 200 cells were counted for each strain. The boxes represent the middle 50% of measurements and the whiskers the minimum to maximum values of the measurements.

Loss of acid-fast staining and altered mycolic acid metabolism in response to pknA and pknB depletion

Based on prior work with a PknA and PknB inhibitor showing broad effects on the mycobacterial cell envelope [6, 19], we investigated whether kinase depletion affected acid fast staining, which results from dye-retention in the abundant mycolic acids and glycolipids in the mycobacterial cell envelope [31]. We observed a marked decrease in acid-fast staining of all 3 strains, first evident at day 4 following onset of kinase depletion, with most cells becoming non-acid-fast by day 6 (Fig 5A). This finding is consistent with progressive alteration of the cell envelope in the absence of PknA and PknB activity.

We therefore investigated whether this phenotype was associated with changes in total or newly synthesized mycolic acids in the kinase replete versus depleted strains. TLC of total mycolates in the depleted strains compared to induced strains did not show changes when normalized to whole cell lipids (S3A Fig). We then used a newly developed method to examine de novo synthesis of mycolates by mass spectrometry of cells grown in the presence of ¹³C acetate [32] (Fig 5B). This assay works on the principle that collision induced dissociation mass spectrometry allows reliable isolation of a precursor molecule in a narrow mass window, which largely removes signals from naturally occurring isotopes, allowing new isotopes from ¹³C labeling to be reliably identified. These experiments showed clear and reproducible decreases in new synthesis of mycolates in the kinase depleted versus kinase replete strains (Fig 5C).

This approach was used to further investigate the degree of isotope labeling of the affected lipids (Fig 5D). We observed consistent decreases in all three depletion strains, though less severe than observed with isoniazid treatment, which completely eliminates mycolic acid synthesis. As a specificity control, we analyzed C26 free fatty acids (m/z 395.39) in the total lipid extracts to evaluate ¹³C incorporation. We observed isotope enrichment for the wild type treated by INH and in all depletion strains compared to the unlabeled wild type (S3B Fig). This result indicates that all strains are able to take up ¹³C acetate, so that the observed differences between the wild type and the depletion strains were specifically due to altered de novo mycolate biosynthesis.

Finally, we examined the abundant mycolyl-glycolipids trehalose monomycolate (TMM) and trehalose dimycolate (TDM) by TLC. In replicate experiments we saw increases of TMM in all three depletion strains, though we did not see consistent changes in TDM (Fig 5E and 5F). The accumulation of TMM in the depletion strains suggests decreased conjugation of TMM to produce TDM by the action of mycolyl transferases FbpA and FbpB. We previously demonstrated that expression of fbpB is repressed by binding of the response regulator MtrA to the fbpB promoter-region and that phosphorylation of Thr213 in MtrA disrupts this binding [6], leading us to propose a mechanism linking decreased MtrA phosphorylation with decreased FbpB-mediated conjugation resulting in accumulation of TMM. Consistent with this mechanism we found decreased expression of fpbB (4.6-fold vs. wild type) and other MtrA-regulated genes in the pknA+pknB depletion strain (Fig 6), where we observed significantly decreased phosphorylation of MtrA both at the previously identified Thr213 residue, and at Thr217, a new phosphorylation site identified in this study. In addition, we found that phosphorylation of Mmpl3, the “flippase” of TMM across the inner mycobacterial membrane [33], is significantly decreased at 2 sites in the pknA depletion strain, which may also affect the levels of TMM and the rate of conjugation to TDM.

Fig 6 — The *pknA*+*pknB* depletion strain was grown with or without 0.25 μg/ml ptc and wild type M. *tuberculosis* was grown without ptc. Cells were harvested at day 4 and RNAs were extracted with Trizol as previous described [56]. RT-qPCR was performed and the data were analyzed using the ΔΔCT method. Biological duplicate experiments were performed with technical replicates in each experiment. Error bars show +/- 1 S.D.

Increased antibiotic susceptibility in the setting of pknA and pknB depletion

Deletion or inhibition of PknB orthologues in Gram-positive bacteria can lead to increased susceptibility to β-lactam antibiotics [34–36], and meropenem has been shown to potentiate the activity of a PknB inhibitor 2-fold in auxotrophic M. tuberculosis [37]. Though carbapenems have been used to treat MDR-TB, cephalosporins and penicillins are generally not active against M. tuberculosis at clinically relevant concentrations. We therefore investigated whether representative β-lactams would have increased activity against the kinase-depleted strains. Under conditions of partial kinase depletion that allowed sufficient growth to test for antibiotic activity, we found that the carbapenems meropenem and faropenem, and the cephalosporins cefotaxime and ceftriaxone were markedly potentiated (2 to 32-fold) for inhibiting growth in the setting of pknA or pknB depletion, with lesser potentiation (1 to 8-fold) when both pknA and pknB were depleted (Table 1). We did not observe increased susceptibility to isoniazid, ethambutol and levofloxacin. Rifampin, however, showed 2-8-fold potentiation in the depletion strains. The differences in potentiation of the different antibiotic classes likely reflect their distinct mechanisms of action, uptake, egress and/or metabolism that are differently affected by altered activity of PknA or PknB. These findings also suggest that an inhibitor of PknB or PknA could be useful in TB treatment regimens both as a direct acting TB drug and as a potentiator of the activity of β-lactam antibiotics and rifampin.

Table 1. Lowest inhibitory concentrations of antibiotics in wild type and kinase depletion strains (μg/ml).

	Faropenem	Cefotaxime	Ceftriaxone	Meropenem	Rifampin	Ethambutol	Isoniazid	Levofloxacin
Wild Type	2–4	4–8	8	1–4	0.0125	1.25	0.16	0.31
pknA depletion	0.25	0.25–0.5	0.25–0.5	0.25–0.5	0.0031–0.0063	ND	0.078	ND
pknB depletion	0.25	2	0.25	0.125	0.0015	0.625	0.078	0.15
pknAB depletion	2	4	4	0.5	0.0015	0.625	0.078	0.15

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ND: Not done.

Because of the up to two-fold overexpression of pknB in the pknA depletion strain when pknB is induced with 20 ng/ml of atc, we performed MABA assays for cefotaxime and meropenem to compare the effects of induction with 10 vs. 20 ng/ml of atc. We observed the same effect of pknA depletion on susceptibility to these two β-lactam antibiotics at both atc concentrations (S4 Fig). We also examined the acid-fast staining phenotype of this strain and found similar effects on this phenotype when the strain was grown in the presence of 10 or 20 ng/ml of atc (S5 Fig). These two results, together with the growth curve data showing nearly identical growth of the pknA depletion strain with either 10 or 20 ng/ml of atc to induce pknB expression (Fig 3C), indicate that these phenotypes result primarily from effects of pknA depletion rather than from differences in pknB expression in this strain.

Differential phosphorylation in kinase depletion strains and candidate substrate identification

We compared phosphorylation in each of the kinase depletion strains grown without inducer, to the same strains in which kinase expression was induced. We obtained samples on day 4 for pknA and pknB single depletion, and day 5 for pknA+pknB double depletion, based on experiments showing the onset of a clear growth defect but limited cell death in the depleted strains at these time points (Figs S6 and 3D). Because depletion occurs over days, differences in protein phosphorylation will reflect both direct effects of PknA and/or PknB depletion and downstream effects that result from changes in the activity of other kinases, as well as possible differences in the rate of depletion of the two kinases or in rates of turnover of individual phosphoproteins. For the pknA depletion only, the up to 2-fold overexpression of pknB may have contributed to the differential phosphorylation results observed with this strain.

Overall, we observed Ser, Thr or Tyr phosphorylation of 712 proteins for which one or more phosphorylation sites were present in at least 8 of 18 samples analyzed in at least 1 strain (S2 Table). Using criteria of log₂FC <-1 and adjusted P <0.05, we identified 302 unique sites in 204 proteins that showed significantly decreased phosphorylation in one or more kinase-depleted strain compared to the same strain with induced kinase expression. We also identified 299 unique phosphorylation sites in 201 proteins that were significantly increased (log₂FC >1, adjusted P <0.05) (S2 Table). While most proteins were phosphorylated on one to three sites, some were phosphorylated on many sites, the most striking examples being FhaA and MmpS3, which are phosphorylated on 23 and 16 unique residues, respectively.

A surprising finding was the large number of phosphorylation sites that showed decreased phosphorylation in the pknA depletion strain, where 234 unique phosphorylation sites in 171 proteins were significantly decreased (Fig 7A and S3 Table). In the pknB depletion strain, phosphorylation was significantly decreased at 52 unique sites in 43 proteins, and in the pknA+pknB depletion strain at 74 unique sites in 60 proteins (Fig 7B and 7C; S3 Table). Confirming specific kinase depletion, phosphorylation was significantly decreased at 6 sites in PknA and none in PknB in the pknA depletion strain, and phosphorylation of 3 sites in PknB and none in PknA were decreased in the pknB depletion strain. In the pknA + pknB depletion strain, two sites in PknA and one site in PknB were significantly decreased.

Fig 7 — **7A, 7B, 7C)** Volcano plots of phosphopeptides in the indicated kinase depletion strains shown as the ratio (Log₂FC) of peptide abundance in the kinase depleted vs. kinase replete strains, as described in the Methods. Significantly differentially abundant phosphopeptides (Log2FC <-1.0 or >1.0 and adj. P<0.05) are shown in red. Data are from 3 biological replicates with 3 separate cultures in each replicate for each strain. **7D)** Venn diagram showing overlap between strains of the proteins from which significantly decreased phosphopeptides in the kinase depleted compared to the kinase replete samples were observed. **7E)** pLogos showing relative statistical significance of amino acids at positions adjacent to the phosphoacceptor Thr in phosphopeptides that were significantly decreased in each kinase depletion strain. The red horizontal line indicates P = 0.05 after Bonferroni correction. Additional pLogos for significantly decreased Ser-centered and for increased Thr and Ser-centered phosphopepetides are in S9 Fig.

Identification of direct substrates of a kinase can provide insight into mechanisms by which protein phosphorylation by that kinase alters cell physiology. The patterns of decreased phosphorylation of specific peptides in the depletion strains allow identification of candidate substrates of PknA and PknB. We identified candidate PknA substrates using criteria of i) significantly decreased phosphorylation with pknA depletion and ii) increased or not significantly changed phosphorylation with pknB depletion. Similarly, significantly decreased phosphorylation with pknB depletion and increased or not significantly changed phosphorylation with pknA depletion identified candidate PknB substrate sequences. Sites with significantly decreased phosphorylation in both the pknA and pknB depletion strains were identified as candidate substrates of both PknA and PknB. Using these criteria we identified 127 candidate PknA substrate sequences, 28 candidate PknB substrate sequences, and 15 candidate PknA + PknB substrate sequences (S4 Table). At the protein level we identified 104 candidate PknA substrates, 23 candidate PknB substrates and 14 candidate PknA + PknB substrates. Given the non-comprehensive nature of phosphoproteomics, and its inability to distinguish direct from indirect effects of kinase depletion, these candidate substrate identifications will need to be tested using targeted approaches. As described below, however, these results clearly identify several pathways required for growth and virulence that are affected by depletion of PknA and/or PknB.

To examine the extent of overlap of the proteins and phosphopeptides with decreased phosphorylation among the three depletion strains we performed Venn diagram and correlation analyses. Of 43 proteins with decreased phosphorylation sites in the pknB depletion strain, 26 (60%) also had decreased sites in the pknA and 15 (35%) in the pknA+pknB depletion strains (Fig 7D). Of 60 proteins with decreased phosphorylation in the pknA+pknB depletion strain, 43 (72%) were decreased in the pknA strain including 14 common to all three strains. At the peptide level, we saw less overlap between the pknA and pknB depletion strains, where 14 of 52 (27%) unique phosphopeptides with decreased phosphorylation in the pknB depletion strain were also decreased in the pknA strain (S7A and S7B Fig). Similarly, among all quantified phosphopeptides, we observed no correlation between abundance of individual phosphopeptides with pknA vs. pknB depletion, or with pknB depletion vs. pknA+pknB depletion. However, there was moderate correlation (r = 0.438) between the pknA and pknA+pknB depletion strains (S7C–S7E Fig). These findings suggest PknA has a very broad role in regulating M. tuberculosis via Ser/Thr phosphorylation, and that while PknA and PknB regulate many of the same proteins and pathways, the manner in which the do so often differs. They also suggest that when both kinases are depleted, differences in Ser/Thr phosphorylation predominantly reflect the loss of PknA activity.

We also compared the phosphoproteins showing decreased phosphorylation in the kinase depletion strains to phosphoproteins that were decreased in a recent study of pknB depletion, as well as to our prior results identifying proteins with decreased phosphorylation in response to a chemical inhibitor of both PknA and PknB [6, 24]. In each case we observed partial overlap between the results from this study and the prior findings (S8A–S8C Fig). We also compared the total quantified phosphoproteins identified in this study to those found in the kinase inhibitor study and to an earlier phosphoproteomic study in which we identified phosphorylation sites in M. tuberculosis at different growth stages and in response to stresses [7]. We found that 69% of all phosphoproteins in the kinase inhibitor study and 43% of all phosphoproteins in stress phosphoproteome study were present in the quantified phosphoproteins identified in the current study (S8D Fig).

We searched the differentially phosphorylated peptides for conserved sequence motifs using the pLogo algorithm [38] and identified distinct patterns in the amino acid sequences surrounding the phosphoacceptor Thr for phosphopeptides with decreased or increased phosphorylation in the pknA, pknB and pknA+pknB depletion strains (Figs 7E and S9). The pLogo for phosphopeptides with significantly decreased Thr phosphorylation in the pknA depletion strain showed significant enrichment of acidic residues at -1, -3 through -6, and at +7; Pro at -1 and +2; hydrophobic residues at +3 (Leu, Val); and basic residues at +4 (Lys, Arg). In the pknB depletion strain we noted enrichment of specific residues at several positions, though none reached statistical significance, likely because of the small number of phosphopeptides analyzed. The motif from the pknA+pknB strain was highly similar to the pknA depletion strain motif. The pknA and pknB depletion strain motifs were less similar, consistent with the limited overlap in phosphopeptides with decreased phosphorylation between these strains (S7A Fig). These motifs also showed similarity to those we identified in phosphopeptides with decreased phosphorylation in the kinase inhibitor experiments, most notably the acidic residues at -3 and Pro at +2 [6].

PknA and PknB target pathways required for growth and virulence

Several processes that are essential for growth were identified in the differential phosphorylation data. Consistent with prior findings, these data include phosphorylation-mediated regulation of peptidoglycan synthesis by PknA and PknB. In the pknA depletion strain we identified significantly decreased phosphorylation of sites in the essential peptidoglycan synthesis regulators Wag31 (DivIVA) and CwlM (S3 Table) [21, 29]. In the pknB depletion data we identified a Thr phosphorylation site with significantly decreased phosphorylation in CwlM and 3 sites with decreased phosphorylation in FhaA, an essential forkhead-associated domain protein that interacts with CwlM and MviN, the essential peptidoglycan flippase. Strikingly, FhaA (15 sites including all 3 sites with decreased phosphorylation in the pknB strain), Wag31 (4 sites), and MviN (2 sites) showed increased phosphorylation in the pknA depletion strains. These findings indicate that PknA and PknB target essential peptidoglycan synthesis regulators to control M. tuberculosis growth, but in distinct ways.

The quantitative phosphoproteomic data also show protein secretion to be a major target of PknA and PknB-mediated phosphorylation. In the pknA depletion strain we identified decreased phosphorylation of three sites in SecE, an essential protein of the general secretion system and 1 site in FtsY, the signal particle receptor of this system. Two of the sites in SecE also showed decreased phosphorylation in the pknB and pknA+pknB depletion strains and a distinct site in FtsY also showed decreased phosphorylation in the pknA+pknB depletion strain. Additionally, four sites in TatA, an essential channel forming protein of the twin-arginine transport system showed decreased phosphorylation in the pknA strain, one of which was also decreased in the pknA+pknB depletion strain, and a distinct TatA site had decreased phosphorylation in the pknB depletion strain. Further, five proteins in the virulence-associated ESX-1 secretion system showed decreased phosphorylation in the pknA depletion strain: one site each in the ESX-1 regulator/nucleoid associated protein EspR, the immunodominant secreted antigen EspF, and the ESX-1-associated protein EspL, as well as four and six unique sites, respectively, in the secreted virulence proteins EspB and EsxB. Three EsxB sites also had decreased phosphorylation in the pknA+pknB depletion strain as did the single EspL site and a distinct EspF site. In the pknB depletion strain, only a unique EsxB site showed decreased phosphorylation. These findings suggest that the three major protein secretion systems of M. tuberculosis are regulated by PknA and/or PknB-mediated protein phosphorylation.

Translation also appears to be extensively regulated by PknA and PknB. In the pknA depletion strain, nineteen ribosomal proteins, the initiation factors InfA and InfC, and the peptide chain release factor-2 PrfB showed decreased phosphorylation. In contrast, initiation factor Tuf and two distinct PrfB phosphopeptides were three of the five phosphopeptides with the greatest increase in phosphorylation in this strain. In the pknB depletion strain, only three ribosomal proteins showed decreased phosphorylation, suggesting a more limited role for PknB in translation control.

Several proteins involved in transcription regulation were significantly differentially phosphorylated in the kinase depletion strains. In the pknA depletion strain, in addition to specific transcription regulators including SmtB, IdeR, RegS3, and PhoP, proteins expected to have broad effects on transcription showed decreased phosphorylation, including the transcript cleavage factor GreA, the primary sigma factor SigA, and the nucleoid-associated proteins HupB (HU) and EspR. In the pknB depletion strain two different proteins with broad effects on transcription, the nucleoid-associated protein Lsr2 and the termination protein Rho, showed significantly decreased phosphorylation. These data point to both targeted and broad regulation of transcription by PknA and PknB, but with distinct roles for each kinase.

Our data also indicate kinase-mediated regulation of cell division and DNA synthesis, key aspects of the bacterial cell cycle. Phosphorylation of FtsQ, an essential protein involved in septum formation, was significantly decreased in the pknA depletion strain but increased in the pknA+pknB depletion strain; several other cell division proteins (FtsB, FtsE, FtsK and FtsZ) were phosphorylated in one or more strains but did not meet our significance criteria. Essential DNA synthesis proteins were also affected, with the single strand DNA binding protein Ssb showing significantly decreased phosphorylation in the pknA depletion strain and the replication initiator protein DnaA showing significantly increased phosphorylation. DnaN, the DNA polymerase III initiator, showed increased phosphorylation just below our change threshold. Significantly decreased phosphorylation of the response regulator MtrA was seen in the pknA+pknB depletion strain. We recently showed phosphorylation-regulated binding of cognate promoter region DNA by MtrA [6], a response regulator that has been implicated in regulating initiation of chromosome replication, peptidoglycan turnover and glycolipid synthesis. Consistent with the prior finding of increased MtrA binding and repression of MtrA-regulated gene expression in the absence of MtrA phosphorylation, we observed decreased expression of MtrA-regulated genes in the pknA+pknB depletion strain, where MtrA shows decreased phosphorylation (Fig 6) [39–41].

Each of the above pathways was targeted in our kinase inhibitor data [6], though in most cases less extensively and often at different sites compared to these kinase depletion data. We also identified new processes affected by PknA and PknB-mediated phosphorylation. A notable example is decreased phosphorylation of several antitoxin proteins of the VapBC and MazEF toxin-antitoxin modules in the pknA depletion data. This result suggests that PknA-mediated signal transduction is a regulatory mechanism for the action of the toxin proteins, which typically degrade specific RNAs resulting in growth arrest, antibiotic and other stress tolerance, and remodeling the M. tuberculosis proteome [42–44].

In summary, these quantitative phosphorylation data identify numerous proteins of core cell physiologies required for growth and virulence as targets of phosphorylation by PknA and PknB (S3 Table, Fig 7). Taken together, these data indicate substantial overlap in pathways targeted by these kinases, but with distinct activities of PknA and PknB.

Discussion

Reversible Ser/Thr and Tyr phosphorylation is increasingly recognized as an important component of transmembrane signal transduction in bacteria. PknB orthologues in Gram-positive bacteria have been shown to regulate peptidoglycan metabolism, morphogenesis and susceptibility to cell wall active antibiotics [34, 35, 45, 46]. In mycobacteria and other actinobacteria, the presence of several transmembrane Ser/Thr kinases indicates an extensive role for protein phosphorylation in regulating cell processes in response to extracytoplasmic signals. In M. tuberculosis, an obligate human pathogen, these kinases must sense and transduce host signals to allow the pathogen to adapt, survive and cause disease.

In previous work we identified proteins in a number of pathways that were differentially phosphorylated in response to treatment with a small molecule inhibitor of both PknA and PknB [6]. In this study, using the titratable ptc-regulated promoter system, we examined the effects of pknA and pknB depletion, individually and in combination, allowing us to focus on the effects of depleting each kinase while avoiding potential off-target effects that can occur with chemical inhibition.

An unexpected result from this work is the greater number of proteins that showed decreased phosphorylation in the pknA depletion strain compared to the pknB depletion strain and the relatively limited overlap in differential phosphorylation between these strains. This finding clearly indicates a major role for PknA in regulating the M. tuberculosis phosphoproteome, but it is possible that limitations of our approach may have affected these results. For example, although we titrated RNA and protein for each kinase at day 4 following onset of depletion, we do not know whether PknA and PknB have different rates of decay of over time. If PknA decays more quickly, for example this might lead to relative underestimation of the number of proteins targeted by PknB compared to PknA. Further, distinct decay rates for individual phosphoproteins may have affected our detection of significantly decreased phosphorylation of some proteins in our experiments. Finally, our use of an atc concentration that results in up to two-fold higher than wild type levels of pknB expression in the pknA depletion strain may have affected the differential phosphorylation that we detected in this strain.

The manner in which PknA and PknB interact functionally is not well understood. Early work showed that PknA and PknB can cross-phosphorylate each other [8], though later data suggested unidirectional trans-phosphorylation of PknA by PknB, leading to the hypothesis that PknB regulates PknA activity [47]. Subsequent work, however, showed that PknA autophosphorylates in the mycobacterial cell and that PknA abundance is unaltered when PknB is depleted [15, 48]. Our data showing limited overlap in peptides that are differentially phosphorylated in the pknA versus pknB depletion strains argue against a pathway in which PknB activates PknA. Rather, our data provide evidence of coordinate regulation of several pathways but with distinct regulatory roles for PknA and PknB, via phosphorylation of different sites in the same protein, or of different proteins within the same or related pathways.

We identified altered phosphorylation in the depletion strains of proteins in several pathways required for growth, suggesting that these kinases broadly adapt M. tuberculosis physiology to regulate growth, as has been shown for PknB in the setting of hypoxia-mediated growth arrest and renewed growth with reaeration [49]. Consistent with our kinase inhibitor data [6], the kinase depletion results also indicate regulation by PknA and PknB of the mycobacterial cell envelope. Decreased phosphorylation of sites in FhaA, and CwlM in the pknB depletion strain support the previously identified role for PknB in regulating peptidoglycan synthesis [6, 10, 21, 24, 50]. In the pknA depletion strain, the differential phosphorylation of the peptidoglycan synthesis regulators Wag31, CwlM and FhaA, and of the MviN peptidoglycan flippase, newly identify a major but distinct role for PknA in regulating peptidoglycan synthesis.

The time-dependent loss of acid-fast staining in the setting of kinase depletion suggests alterations in the lipid content and/or structure of the mycobacterial envelope, particularly the outer membrane. We observed decreased de novo mycolic acid synthesis in the kinase depletion strains and accumulation of TMM, consistent with decreased mycolate conjugation to produce TDM. Though levels of TDM did not clearly change in our TLC analysis, our prior kinase inhibitor study showed small decreases in TDM following kinase inhibition and demonstrated decreased abundance of the mycolyl transferase protein FbpB (antigen 85b) [6]. Here we determined that fbpB expression, which is repressed by binding of unphosphorylated MtrA, is markedly reduced in the pknA+pknB depletion strain. Together, these results provide a candidate mechanism for our findings, i.e. that decreased FbpB activity in the setting of kinase depletion contributes to accumulation of TMM, despite decreased de novo mycolate synthesis. Changes in mycolate conjugation also suggest a possible mechanism for decreased acid-fast staining. However, the stable levels of total saponifiable mycolates suggest that the location and membrane configuration of mycolyl lipids are relevant, and that additional mechanisms may play a role, potentially including phosphorylation-dependent changes in MmpL3 flippase activity or changes in the structure of other components of the cell envelope that alter dye retention.

Differential phosphorylation of transmembrane protein secretion pathways, including the general secretory and twin arginine transport pathways, as well as Type VII secretion systems, particularly the virulence-associated ESX-1 pathway, further highlights targeting of the pathogen-host interface by PknA and PknB. Individually depleting pknA or pknB, for example, led to decreased phosphorylation of the same two sites in SecE, a component of the general secretory translocon, indicating that both kinases regulate this secretion system. In contrast, four sites in TatA showed decreased phosphorylation only in the pknA depletion stain. In the ESX-1 system pknA depletion led to decreased phosphorylation of the EspR regulatory protein, the ESX-1 associated protein EspF, whereas the secreted substrate protein EsxB (Cfp10) showed decreased phosphorylation, though at distinct sites, in the pknA and the pknB depletion strains.

In addition to cell envelope structure and functions, we identified several other core pathways that are essential for growth and viability that are differentially phosphorylated in the kinase depletion strains. These include translation, where 18 and 3 ribosomal proteins showed decreased phosphorylation with pknA and pknB depletion, respectively. Several transcription proteins also showed decreased phosphorylation in the pknA strain and pknB depletion strains, including transcription factors that specifically regulate small sets of genes, as well as proteins that have broad, genome scale activities. These include the primary sigma factor SigA, the nucleoid-associated proteins MihF and HupB, and the transcription elongation factor GrpE in the pknA depletion strain, and the nucleoid-associated protein Lsr2 and the transcription termination protein Rho in the pknB depletion strain. These findings suggest broad targeting of transcription by both kinases, but via differential phosphorylation of distinct proteins.

The differential phosphorylation of key regulators of peptidoglycan synthesis and the changes in mycolate synthesis and conjugation in the kinase depletion strains provide candidate mechanisms for the increased antibiotic susceptibility that we observed. The marked increased susceptibility to β-lactams of both single depletion strains, and to a lesser extent the pknA + pknB strain, mirrors similar observations with deletion of pknB orthologues in Gram-positive bacteria where this gene is not essential [34]. Though the specific changes in peptidoglycan resulting from kinase depletion or inhibition have not been determined, the data from this study, together with our prior transcriptomic and metabolomic data showing a block in diaminopimelate synthesis in the setting of kinase inhibition [6], suggest effects on peptidoglycan precursor availability that could lead to incomplete or abnormal peptidoglycan structure and increased susceptibility to β-lactam antibiotics. The effects of pknA and pknB depletion and inhibition on mycolate metabolism suggest a mechanism by which alterations in the lipid and glycolipid components of the cell envelope could enhance entry of the highly lipophilic drug rifampin to achieve increased concentrations and activity in the M. tuberculosis cell. We identified one site (Thr764) in the beta subunit of RNA polymerase, the protein in which nearly all rifampin resistance conferring mutations occur, that showed increased phosphorylation in the pknA depletion strain. Though this site is not in the rifampin resistance determining region where most resistance mutations occur [51], it is possible that phosphorylation of this site might affect rifamycin susceptibility.

The magnitude of decreases in lowest inhibitory concentrations of the β-lactams in the kinase-depleted strains suggests that kinase inhibitor-β-lactam combinations have the potential to be clinically useful additions to anti-tuberculosis therapy, both for their direct effects and for their potentiation of β-lactam activity. Current research trials are testing increased rifampin doses to accelerate M. tuberculosis killing and shorten tuberculosis treatment. The magnitude of the potentiation of rifampin activity in all three depletion strains suggests that kinase inhibitor-rifamycin combination could be a valuable component of anti-tuberculosis therapy by enhancing rifamycin activity in vivo without the increased toxicity associated with higher rifampin dosing.

In addition to identifying multiple essential pathways targeted by PknA and PknB, our findings lead to several questions regarding the role of these kinases in M. tuberculosis physiology. One area of particular interest is how PknA and PknB interact functionally. The phosphoproteomic data in this study suggest a broad role for PknA with a more limited, partially overlapping, but essential role for PknB, with both kinases targeting pathways required for growth and viability. That pknA and pknB are co-expressed from adjacent translationally coupled genes strongly suggests that these two kinases act in a coordinated manner. However, the limited overlap in differential phosphorylation and the presence of a transcription start site positioned to transcribe pknB alone [52], indicate that PknA and PknB likely act independently under some conditions. A second priority area, identification of direct in vivo substrates of PknA and PknB, will further inform the specific functions of each protein. Our differential phosphorylation data identifying candidate substrates for both kinases (S4 Table) provide a valuable resource for investigating how PknA and/or PknB-mediated phosphorylation affects protein function. These insights will be essential for understanding how these kinases contribute to tuberculosis pathogenesis and how they may be targeted to enhance anti-tuberculosis therapy.

Materials and methods

Strains, media, reagents, plasmids and primers

M. tuberculosis H37Rv was used as the wild type and as the parental strain for all mutants. E. coli TOP10 and XL1 blue were used for cloning and were grown in LB broth supplemented with appropriate antibiotics (25 μg/ml kanamycin or 150 μg/ml hygromycin). M. tuberculosis H37Rv was grown at 37°C in Middlebrook 7H9 liquid medium (Difco) supplemented with 0.5% albumin, 0.2% glucose, 0.085% NaCl, 0.2% Glycerol and 0.05% Tween 80 (7H9-ADN-Tw). Kanamycin (25 μg/ml) or hygromycin (50 μg/ml) was added to liquid or agar medium when appropriate. Restriction endonucleases and DNA modifying enzymes were purchased from New England Biolabs. Pristinamycin 1A (ptc) was purchased from Molcan Corporation (Canada). Anti-phosphothreonine antibody and Anti-rabbit IgG HRP-linked antibody were purchased from Cell Signaling Technology. Analytical grade chemicals and reagents were purchased from Sigma Aldrich. Details of primers, plasmids and strains are shown in S1 Table.

Generation of conditional depletion strains in M. tuberculosis

Plasmid pMYT797 was kindly provided by Francesca Forti [25]. The pknB depletion strain was constructed through introducing pMYT797 into the M. tuberculosis chromosome by a single homologous recombination event as previous described [25] (S1A Fig). To generate the pknA + pknB conditional depletion strain, pMYT797 was digested by SphI, blunted, and subsequently NcoI digested. A 770 bp 5’ region of pknA (+1 to +770bp) was PCR-amplified using primers JZ-1 and JZ-2. The PCR product was digested by NcoI and NruI, and ligated into pMYT797. The targeting construct thus obtained was electroporated into M. tuberculosis H37Rv. Candidate single cross-over transformants were obtained by hygromycin selection. PCR of genomic DNA from candidate strains, using primers annealing to flanking regions of pknA-pknB, yielded the expected 4kb PCR product, which was confirmed by sequencing. The Psmyc of pTE-28S15-0X was fused to tetR of pTE-10M-0X and cloned into PacI and ClaI sites of pTC-0X-1L [53]. To obtain the pknA depletion strain from the pknA+pknB depletion strain, pknB was PCR-amplified using primers JZ-5 and JZ-6 and cloned under control of the uv15tetO promoter in pTC-0X-1L-psmyc-tetR (pRH2522) [54] and transformed into the pknA+pknB depletion strain. Growth of this strain in the presence of anhydrotetracycline (atc) induces expression of pknB, resulting in the pknA depletion strain (S1B Fig).

Analysis of inducible gene expression by fluorescence-activated cell sorting (FACS) assays

To investigate whether the ptc inducible promoter (PIP) system can be titrated at the single cell level, we used FACS to compare mCherry expression regulated by the NitR-controlled promoter, to expression regulated by pptr. mCherry was cloned into the pNit vector and pRH2046, and the resulting plasmids were electroporated into the M. tuberculosis auxotroph mc2-6206 (H37Rv ΔleuD ΔpanCD::hyg) [26, 55]. The transformants were selected by kanamycin for pNit-mCherry and spectinomycin for pRH2046-mCherry. Individual colonies were inoculated into 7H9+ADN+leucine+pantothenate and grown for 3 weeks. The bacteria were diluted to an optical density at 600nm of 0.2 and ptc was added at 0.576 μM, 0.072 μM, 0.009 μM, 0 μM, or isovaleronitrile was added at 2.5 μM, 0.63 μM, 0.08 μM, 0 μM. Cells were harvested after inducing 48 h, resuspended in PBS and filtered through a 35-um cell strainer (Cat# 352235, BD Falcon). Single cell fluorescence was determined using a MACSQUANT-VYB flow cytometer (Miltenyi Biotec, Bergisch Gladbach, Germany). Analyses were carried out using FlowJo Software.

Assessing gene essentiality for replication and viability

To characterize the growth of the depletion mutants, the strains were grown to logarithmic phase (OD₆₀₀ 0.8–1.0) in the presence of ptc. Cells were collected, washed, and then diluted to OD₆₀₀ of 0.03–0.05 in 7H9-ADN-Tw with or without ptc. Two-fold dilutions of ptc were added from 1 μg/ml to 0.0039 μg/ml, cultures were incubated at 37°C with shaking and the OD₆₀₀ was recorded daily. Wild type H37Rv was cultured in parallel. Experiments were performed in triplicate, and the average OD₆₀₀ values were plotted as a function of time. Cultures were also grown with and without ptc induction, washed and then plated at serial time points on Middlebrook 7H9 agar with ptc induction to assess for viability of each depletion strain over time (S1C Fig).

RNA and total protein extraction

For analysis of pknA and pknB expression in the depletion mutants, strains were grown with different ptc concentrations as described above. Samples from M. tuberculosis cultures were taken at day 4 and day 6 for isolation of RNA and total protein. Cells were harvested and treated as previously described [56]. RNA was extracted, purified and treated with Dnase I and Turbo DNAse (Thermo Fisher). Quantity and quality of RNA was determined using a Nanodrop instrument (Thermo Fisher). Proteins were extracted and solubilized in 9.5 M urea/2% CHAPS, pH 9.1.

RT-qPCR

Reverse transcription (RT) and quantitative PCR of cDNA (RT-qPCR) were performed on equal amounts of total RNA for each condition, using the Quanta Biosciences qScript cDNA synthesis kit and the PerfeCTa-SYBR green Supermix, respectively as previously described [56]. Data were analyzed using the ΔΔCT method with M. tuberculosis sigA as the reference gene [57]. Biological duplicate samples were analyzed for each target sequence.

Western blotting

For western blotting, 10 μg of total protein for each condition was resolved by SDS-PAGE followed by transfer to a PVDF membrane, which was probed with primary rabbit polyclonal antibodies against PknA (1:3,000) or mouse monoclonal antibody against PknB (1:3000) [18] followed by HRP-linked anti-rabbit secondary antibody (1:10 000) (Cell Signaling Technology). The blot was incubated with LumiGLO chemiluminescent substrate (Cell Signaling Technology) and detected using a Kodak Image Station (Carestream Health).

Acid-fast staining

Strains were grown in 7H9-ADN-Tw medium, pelleted and fixed in 4% paraformaldehyde in PBS for 2 h. Fixed cells were pelleted, washed and resuspended in PBS. 10 μl of cell suspension were spread onto a glass slide. The slides were heated, stained with the TB Carbolfuchsin KF kit (Becton Dickinson) following the manufacturer’s instructions. Slides were rinsed with distilled water, dried, and 10 μl of Cytoseal 60 was placed on top of the sample and covered with a coverslip. A Nikon Eclipse 55i microscope was used to observe acid fast stained cells.

Microscopy

For examination of cell morphology, cells were grown, harvested, fixed and resuspended in PBS as described above. 10 μl of the cell suspension was spotted on a 1% agarose coated glass slide to immobilize the cells. A coverslip was placed on the slide and cell morphology was visualized using a Nikon Eclipse TE2000-E inverted microscope fitted with a 100x Plan Achromatic phase contrast oil-immersion objective. Photographs were collected with a Hamamatsu ORCA-AG CCD camera, using IPLab imaging software. Measurement of cell length was performed using ImageJ software. Cell length in wild type, pknA depletion, pknB depletion and pknA+pknB depletion strains, with and without ptc induction, were compared using an unpaired t-test, using GraphPad Prism Software.

Microplate Alamar Blue assay (MABA) for antibiotic susceptibility testing

M. tuberculosis pknA, pknB, pknA+pknB depletion strains were grown with ptc induction at 37°C in 7H9-ADC-Tw to OD₆₀₀ ~0.8. The cells were pelleted, washed twice with 7H9-ADC-Tw and diluted to a calculated OD₆₀₀ 0.001 in7H9-ADC-Tw. Each strain was placed in a 96-well clear bottom plate with 2-fold dilutions of ptc across the columns and 2-fold dilutions of antibiotic across the rows. Dilutions were made in 7H9-ADC-Tw and all wells contained 10 μg/ml clavulanic acid. Plates were incubated for 7–8 days and inhibitory concentrations of antibiotics were determined as previously described, based on color change following overnight incubation after addition of Alamar Blue, with pink indicating growth and blue indicating absence of growth. The lowest antibiotic concentration that results in absence of growth (blue well) correlates with minimal inhibitory concentrations obtained using traditional methods [58]. For the kinase depletion strains, we defined the lowest inhibitory concentration as the lowest antibiotic concentration that inhibited growth at the lowest ptc concentration that resulted in full growth in the absence of antibiotic. Biological duplicates were performed for each experiment.

Protein sample preparation, mass spectrometry and phosphopeptide identification

Samples for phosphoproteomics were obtained from 3 independent experiments, in each of which 3 separate cultures with kinase depletion and 3 separate cultures without kinase depletion were grown and processed for phosphoproteomics as described below, resulting in 9 kinase-induced and 9 kinase depleted samples per strain that were treated as independent samples for calculation of differential abundance and P values. Protein samples were prepared as previously described [6, 59]. One mg protein samples were reduced with DTT and transferred into a 30 kDa MWCO ultrafiltration filter (Microcon-30 Centrifugal Filters, Millipore) for filter-aided sample processing [59], followed by washing with ABC solution (8 M Urea in 50 mM ammonium bicarbonate) and alkylated with 0.05 M iodoacetamide solution (IAA, TCI America). Trypsin digestion was carried out using a 1:50 enzyme to protein ratio with sequencing grade modified trypsin (Promega) by incubating at 37°C for 18 hours. The samples were desalted on an Oasis HLB column (1cc, Waters). The desalted samples were eluted with gradient acetonitrile: 500ul 30% ACN in 0.1% FA, then 300ul 50% ACN in 0.1% FA, at last 300ul 70% ACN in 0.1% FA. Samples were ready for phosphopeptide enrichment after evaporating to dryness overnight (Vacufuge plus, Eppendorf).

Phosphopeptide enrichment was performed using a FeCl₃-charged ProPAC IMAC-10 column (Thermo Scientific) as described previously [6]. Phosphopeptide-enriched fractions were desalted using a 1 cc Oasis HLB cartridge (Waters Corp.). Samples were analyzed on a nanoflow ultrahigh-performance liquid chromatography (UPLC) system (400 Series, Eksigent/Sciex) hyphenated with a quadrupole-Orbitrap mass spectrometer (Q Exactive; Thermo Scientific). The Q Exactive mass spectrometer was run in positive-ion mode. Full scans were carried out at a resolution of 70k with an automatic gain control (AGC) target of 3 x 10⁶ ions and a maximum injection time of 120 ms, using a scan range of 375 to 1400 m/z. For tandem MS (MS/MS) data acquisition, a normalized collision energy value of 27 was used. Scans were carried out at a resolution of 17.5 K with an AGC target of 5 x 10⁴ ions and a maximum injection time of 100 ms. The isolation window was set to 1.6 m/z. An underfill ratio of 2.0% was set and a dynamic exclusion value of 25.0 s applied.

The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD015197. RAW files generated using XCalibur software (version 2.2; Thermo Scientific), were analyzed using MaxQuant (version 1.5.6.5) for identification and quantification of phosphopeptides [60]. Phosphorylation (STY) and oxidation (M) were used as variable modifications and carbamidomethylation as a fixed modification. The “match between run” and “label-free quantification (LFQ)” options were enabled. Search was delimited to the M. tuberculosis H37RV version 2 protein sequence database, downloaded from PATRIC [61]. Finally, the evidence file was used to extract the identified peptides. Using the modified sequence column, the average intensity of each modified peptide was calculated.

Differential abundance testing of phosphopeptides between M. tuberculosis strains

Differential abundance testing of induced versus uninduced kinase expression was done for each strain using the metagenomeSeq package in R [62]. Within each strain, only phosphopeptides with non-zero in intensities in 8 or more out of 18 samples (17 samples for pknA depletion because one sample was excluded at the data analysis stage because of poor data quality) were considered for abundance testing. When the same peptide sequence with the same phosphorylation site was detected but differed in either charge state, acetylation, or oxidation, we picked a representative peptide with the highest mean intensity (after median normalizing by sample) across all samples; this representative peptide is shown in the “cleanseq” column in the supplemental tables. The original intensities were then median normalized by sample using only phosphopeptides that met these criteria. For each phosphopeptide, log₂ fold-change (log₂FC) values for the depletion versus control samples were then calculated by metagenomeSeq, controlling for batch effects. The Pearson correlations of log₂ fold-change values between strains were computed using phosphopeptides with log₂ fold-change values estimated in both strains.

pLogo generation

For each of the kinase-depleted data sets, significantly increased or decreased Ser- and Thr-containing phosphopeptides were mapped onto the UniProt M. tuberculosis proteome and extended with adjacent sequence information where necessary. This generated aligned foreground data sets of 15-mers in which the Ser/Thr phosphorylation sites were always located at the central (0) position. Duplicate sequences, sequences with phosphorylated residues within 7 residues of a protein terminus, and sequences for which there exist multiple possible mappings onto the M. tuberculosis proteome were discarded. The aligned background data set of 15-mers was generated by all Ser and Thr residues in the M. tuberculosis proteome with their ±7 flanking residues.

These foreground and background data sets were used as inputs to analyze the phosphorylation motifs corresponding to each kinase using the pLogo tool (http://plogo.uconn.edu) [38]. pLogos depict residues proportionally to the log-odds binomial probability of each residue at each position given the residue frequencies in the background. Overrepresented residues are drawn above and underrepresented residues are drawn below the x-axis. The most statistically significant residues are drawn closest to the x-axis, and a red horizontal bar denotes the 0.05 significance level following Bonferroni correction. pLogos centered at Thr and Ser were generated for each data set, except for the decreased Ser-phosphorylated peptides in the pknA+pknB depletion strain and the increased Ser-phosphorylated peptides in the pknB depletion strain, for which there were too few differentially expressed Ser-phosphorylated peptides to analyze.

Total lipid extraction, cell wall-bound mycolic acids (MA) isolation, and thin-layer chromatography (TLC)

M. tuberculosis wild type and all three depletion mutants were cultured in 7H9+ADN medium to early exponential phase. Bacteria were then harvested according to published methods [32]. Briefly, pellets were washed three times in 10 ml distilled water and then resuspended in 15 ml methanol/chloroform (2:1) for 24 hours for sterilization and lipid extraction. The extractable lipids were separated from the cell pellet by centrifugation. The extraction was repeated with another 15 mL of methanol/chloroform (1:2) for one hour. The extracts were combined as the total lipids. The total lipid profiles were analyzed by TLC (Silica Gel 60, Macherey-Nagel) using a solvent system of chloroform/methanol/water (60/16/2). For the cell wall bound mycolic acids, the delipidated cell wall was saponified as previously described [63]. For TLC analysis, the saponified mycolic acids were methylated to yield mycolic acid methyl esters (MAMEs), which were developed three times with a solvent system of hexanes /diethyl ether (90/15) and developed with 8% (v/v) phosphoric acid and 3% (w/v) cupric acetate and charring.

¹³C labeling of M. tuberculosis mycolic acids and mass spectrometry analysis

For ¹³C labeling, culture media was supplemented without or with 3 mg/ml of [1, 2-¹³C] acetate for 48 hours prior to harvest. Total lipids (for TLC analysis) and cell wall-bound mycolic acids were isolated from the delipidated cell walls as described above. Mycolic acids were analyzed by HPLC-ESI-MS (Agilent 6530 Accurate-Mass Q-TOF and 1260 series HPLC system using a normal phase Varian MonoChrom Diol column) [64]. The isocratic HPLC mobile phase solvents were hexane/isopropyl alcohol (70:30 (v:v)), supplemented with formic acid 0.1% (v:v) and ammonium hydroxide 0.05% (v:v) and samples were run at 0.15 ml/min for 10 min. Collision-induced dissociation mass spectrometry (CID-MS) was carried-out with a collision energy of 40 V and the isolation width was set to 4 m/z. For the ¹³C labeled sample, the precursor ions located in the labeling area 1192 m/z was chosen for the CID-MS.

Supporting information

S1 Table. Primers, vectors and bacterial strains.

(XLSX)

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S2 Table. Total and unique phosphoproteins and phosphopeptides.

All quantified phosphopeptides (phosphopeptides present in at least 8 of 18 samples for each strain) and the proteins that contain these peptides.

(XLSX)

Click here for additional data file.^{(309.3KB, xlsx)}

S3 Table. Significant differential phosphorylation in each strain.

Phosphopeptides that have significantly increased or decreased phosphorylation in each kinase depletion strain.

(XLSX)

Click here for additional data file.^{(76.5KB, xlsx)}

S4 Table. Differentially phosphorylated peptides and candidate substrate assignment.

Candidate PknA substrate phosphorylation sites are defined as showing significantly decreased phosphorylation in the pknA depletion strain and either no significant change or significantly increased phosphorylation or phosphorylation in the pknB depletion strain. Candidate PknB substrate phosphorylation sites are defined as showing significantly decreased phosphorylation in the pknB depletion strain and either no significant change or significantly increased phosphorylation or phosphorylation in the pknA depletion strain. Candidate substrate phosphorylation sites of both PknA and PknB are defined as showing significantly decreased phosphorylation in both the pknA depletion strain and the pknB depletion strain.

(XLSX)

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S1 Fig. Strain construction and expression of pknA and pknB in the pknA, pknA+ pknB, and pknB depletion strains.

S1A) Schematic representation of the kinase depletion mutant construction. The pknA+pknB depletion strain was obtained by single crossover homologous recombination of the amino-terminal region of pknA under control of the pristinamycin (ptc)-inducible pptr promoter into the chromosomal copy of pknA [25]. The position of pknB immediately 3’ of pknA, places both pknA and pknB under control of pptR. To obtain a pknA only depletion strain, pknB under the control of a TetR-regulated promoter [53] was integrated at separate site (mycobacteriophage L5 attB site [65]) in the pknA+pknB depletion strain, so that pknB expression can be induced with atc. Recombination of the 5’ region of pknB under control of the pptR promoter into the chromosomal copy of pknB was used to create a strain in which pknB alone can be depleted. S1B) A tetracycline repressor (TetR)-regulated copy of pknB was introduced into the pknA+pknB conditional depletion strain. In the absence of ptc or atc induction both pknA and pknB are depleted, whereas induction with ptc induces expression of both genes. When atc is added in the absence of ptc, only pknA is depleted. S1C) Wild type, pknA+pknB depletion, pknB depletion and pknA depletion strains were grown in broth and plated on 7H9 agar with or without ptc and atc as indicated and incubated for 3 weeks, demonstrating the absence of growth when pknA alone, pknB alone or both pknA and pknB are depleted.

(TIF)

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S2 Fig. Viability over time of wild type and kinase depletion strains.

Each strain was grown in Middlebrook 7H9-ADN-Tw broth to OD₆₀₀ = 0.6, diluted back to OD₆₀₀ = 0.02, followed by growth in medium with and without ptc or atc as indicated. Serial dilutions were plated at days 4, 8 and 12 colonies were counted after 3 weeks incubation. Day 0 counts were not available for this experiment, but the available data show decreased CFU from days 4 to 12 for each depletion strain.

(TIF)

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S3 Fig. Analysis of total and newly synthesized fatty accids in wild type and kinase depletion strains.

S3A) Thin layer chromatography of saponified cell wall mycolic acids of M. tuberculosis from wild type and kinase depletion strains. M. tuberculosis (wild type), pknA, pknB, pknA+B depletion strains were grown with 0.25 μg/ml pristinamycin at 37°C in 7H9+AND+tw until they reached an OD₆₀₀ of 0.8. The cells were spun down, washed with PBS-Tx (PBS plus 0.05% tyloxapol), and diluted to an OD₆₀₀ of 0.1, and then grown +/-0.25 μg/ml pristinamycin in 7H9+AND without tween-80. At each time point, cells were pelleted and washed with PBS. Biological duplicate samples for TLC were harvested at serial time points by resuspending the bacteria into 15 mL 1:2 (V:V) chloroform:methanol to sterilize samples and extract lipids. Total mycolates were isolated by saponification as previously described [32], dried down under nitrogen and analyzed by TLC (Silica Gel 60, Macherey-Nagel) using 3 developments with 90:15 (v/v) hexane: diethyl ether and developed with 8% (v/v) phosphoric acid and 3% (w/v) cupric acetate and charring. S3B) Detection of newly synthesized fatty acids using ¹³C labeling of wild type and kinase depletion strains. HPLC-MS negative ion mode analysis of the free fatty acid (m/z 395.39) was performed on total lipid extracts from the wild type and kinase depletion strains to measure ¹³C incorporation from [1,2-¹³C] acetate uptake. The ¹³C labeling gave additional peaks from m/z 400 to 415 for all strains. The total lipid extracts used here were also used in experiments shown in Fig 5E and 5F [64].

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S4 Fig. Potentiation of cefotaxime and meropenem in the pknA depletion strain with pknB expression induced by two different atc concentrations.

Inhibitory concentrations of cefotaxime and meropenem in wild type and in the pknA depletion strain were determined using the MABA assay as described in the Materials and Methods section. Experiments were performed with pknB expression induced by either 10 or 20 ng/ml of atc. S4A) Representative images of wild type and pknA depletion strains tested for meropenem susceptibility. The - and + signs above the columns at the left side of each image indicate negative control (medium) and positive control (growth in the absence of antibiotic), respectively. The numbers in red indicate the ptc concentration in μg/ml in each row or column. The yellow circle in the positive control column in the lower panels indicates the well with the lowest ptc concentration that allowed growth in the absence of antibiotics (0.008 μg/ml ptc). The yellow circle in the 0.008 μg/ml column on the right side of the lower images indicates the lowest meropenem concentration that inhibited growth at this ptc concentration. S4B) Summary table of results for wild type and pknA depletion strains with cefotaxime and meropenem. The lowest inhibitory concentration in the pknA depletion strain is the same when 10 or 20 ng/ml of atc is used and is shown as ≤ 0.25 μg/ml because this was the lowest concentration of antibiotics included in these experiments. Biological duplicate experiments were performed for all strains.

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S5 Fig. Acid-fast staining of the pknA depletion strain with pknB expression induced by two different atc concentrations.

Acid-fast staining was performed as described in the materials and methods section on wild type and pknA depletion strains grown in Middlebrook 7H9 medium. The pknA depletion strain was grown in the presence of ptc to induce expression of both pknA and pknB, or in the presence of 10 ng/ml or 20 ng/ml atc to induce the expression of pknB but not pknA. The wild type and ptc-induced pknA depletion strain show acid fast staining of most cells (bright pink), while most cells the pknA depletion strain grown in the presence of 10 or 20 ng/ml of atc are not acid-fast and stain blue with the counterstain.

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S6 Fig. Growth curves of M. tuberculosis (wild type), pknAB depletion, pknB depletion mutant, pknA depletion mutant used for phosphoproteomic analyses.

The cells were grown with 0.25 μg/ml pristinamycin until they reached an OD₆₀₀ of 0.8. The cells were spun down, washed with PBS-Tx (PBS plus 0.05% tyloxapol), and diluted to an OD₆₀₀ of 0.1, and then grown +/- 0.25 μg/ml pristinamycin in 7H9+AND, plus induction of pknB by 20ng/ml atc for pknA depletion. OD₆₀₀ measurements were performed every 24h. Note the slower onset of growth arrest for the pknB depletion strain in these cultures with a 10-fold higher inoculum compared to Fig 3. Data are the average of three biological replicates and error bars represent +/- 1 SD.

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S7 Fig. Comparison of differential abundance of phosphopeptides from the three depletion strains.

S7A. Venn diagram of the significantly decreased unique phosphopeptides identified in each strain. S7B. Venn diagram of the significantly increased unique phosphopeptides identified in each strain. S7C-E. Pearson correlation analysis of Log₂FC of phosphopeptides in kinase-depleted relative to kinase replete bacteria from each of the three depletion strains. Each dot represents a phosphopeptide that was quantified in both of the strains being compared S7C. pknA Log₂FC vs. pknB Log₂FC, S7D. pknB Log₂FC vs. pknAB Log₂FC, S7E. pknA Log₂FC vs. pknAB Log₂FC.

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S8 Fig. Venn diagrams of overlap between phosphoproteins from the current and prior studies.

S8A) Overlap of the decreased phosphoproteins identified in pknB depletion data from this study (N = 43, red) compared to phosphoproteins with decreased phosphorylation identified in pknB depletion data from Turapov et al (N = 13, blue) [24]. S8B) Overlap of the decreased phosphoproteins identified in the pknA+pknB depletion data from this study (N = 60, red) compared to decreased phosphoproteins identified in response to treatment with a small molecule chemical inhibitor of both PknA and PknB (Carette, N = 48, blue) [6]. S8C) Overlap of the decreased phosphoproteins identified in this study in pknA depletion (N = 171, red), pknB depletion (green, n = 43), and pknA+pknB depletion (yellow, n = 60) strains compared to proteins showing decreased phosphorylation in response to small molecule inhibition of PknA and PknB in Carette, et al (N = 48, blue). S8D) Overlap of all quantified phosphoproteins in this study (N = 712, blue) compared to two prior phosphoproteomic studies from this laboratory, Carette 2018 (all quantified phosphoproteins, N = 417, red), and a study that identified phosphorylation sites in M. tuberculosis proteins at different growth stages and following exposure to stresses (Prisic 2010, N = 301, green) [6, 7].

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S9 Fig

S9A, pLogos showing relative statistical significance of amino acids at positions adjacent to the phosphoacceptor Ser in phosphopeptides that were significantly decreased in the pknA or pknB depletion strains. S9B, pLogos showing relative statistical significance of amino acids at positions adjacent to the phosphoacceptor Thr in phosphopeptides that were significantly increased in each kinase depletion strain. S9C, pLogos showing relative statistical significance of amino acids at positions adjacent to the phosphoacceptor Ser in phosphopeptides that were significantly increased in pknA or pknA+pknB kinase depletion strain (there were too few increased Ser-phosphorylated peptides in the pknB-depletion strain to analyze). The red horizontal line indicates P = 0.05 after Bonferroni correction.

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Acknowledgments

The authors thank the members of their laboratories for helpful discussions during the course of this research.

Data Availability

The mass spectrometry proteomics raw data have been deposited to the PRIDE Partnership repository of the ProteomeXchange Consortium with the dataset identifier PXD015197. Reviewer account details: Username: reviewer53879@ebi.ac.uk Password: W6KDECxj.

Funding Statement

This research was supported by National Institutes of Health (https://www.nih.gov/) grants RO1 AI099204 and R21 AI30407 to RNH, R01 AI049313 to DBM, and 1K25HL140186 to JP. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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Associated Data

This section collects any data citations, data availability statements, or supplementary materials included in this article.

Supplementary Materials

S1 Table. Primers, vectors and bacterial strains.

(XLSX)

Click here for additional data file.^{(15KB, xlsx)}

S2 Table. Total and unique phosphoproteins and phosphopeptides.

All quantified phosphopeptides (phosphopeptides present in at least 8 of 18 samples for each strain) and the proteins that contain these peptides.

(XLSX)

Click here for additional data file.^{(309.3KB, xlsx)}

S3 Table. Significant differential phosphorylation in each strain.

Phosphopeptides that have significantly increased or decreased phosphorylation in each kinase depletion strain.

(XLSX)

Click here for additional data file.^{(76.5KB, xlsx)}

S4 Table. Differentially phosphorylated peptides and candidate substrate assignment.

(XLSX)

Click here for additional data file.^{(141.6KB, xlsx)}

S1 Fig. Strain construction and expression of pknA and pknB in the pknA, pknA+ pknB, and pknB depletion strains.

(TIF)

Click here for additional data file.^{(2MB, tif)}

S2 Fig. Viability over time of wild type and kinase depletion strains.

(TIF)

Click here for additional data file.^{(1.8MB, tif)}

S3 Fig. Analysis of total and newly synthesized fatty accids in wild type and kinase depletion strains.

(TIF)

Click here for additional data file.^{(1.3MB, tif)}

S4 Fig. Potentiation of cefotaxime and meropenem in the pknA depletion strain with pknB expression induced by two different atc concentrations.

(TIF)

Click here for additional data file.^{(4.4MB, tif)}

S5 Fig. Acid-fast staining of the pknA depletion strain with pknB expression induced by two different atc concentrations.

(TIF)

Click here for additional data file.^{(4.6MB, tif)}

S6 Fig. Growth curves of M. tuberculosis (wild type), pknAB depletion, pknB depletion mutant, pknA depletion mutant used for phosphoproteomic analyses.

(TIF)

Click here for additional data file.^{(1.6MB, tif)}

S7 Fig. Comparison of differential abundance of phosphopeptides from the three depletion strains.

(TIF)

Click here for additional data file.^{(1.2MB, tif)}

S8 Fig. Venn diagrams of overlap between phosphoproteins from the current and prior studies.

(TIF)

Click here for additional data file.^{(1.8MB, tif)}

S9 Fig

(TIF)

Click here for additional data file.^{(2.3MB, tif)}

Data Availability Statement

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PERMALINK

Protein kinases PknA and PknB independently and coordinately regulate essential Mycobacterium tuberculosis physiologies and antimicrobial susceptibility

Jumei Zeng

John Platig

Tan-Yun Cheng

Saima Ahmed

Yara Skaf

Lakshmi-Prasad Potluri

Daniel Schwartz

Hanno Steen

D Branch Moody

Robert N Husson

Roles

Abstract

Author summary

Introduction

Results

Conditional expression of pknA, pknB and pknA+pknB

Fig 1. FACS analysis of titration of pptr-regulated gene expression.

Fig 2. Titration of kinase expression in the depletion strains.

Growth and cell morphology effects of kinase depletion

Fig 3. Growth of kinase depletion strains.

Fig 4. Morphology of wild type M. tuberculosis cells and cells from the pknA, pknB or pknA+pknB depletion strains.

Loss of acid-fast staining and altered mycolic acid metabolism in response to pknA and pknB depletion

Fig 5. Acid-fast staining and mycolic acid synthesis in pknA, pknB and pknA+pknB depletion mutants.

Fig 6. Relative quantification by RT-qPCR of MtrA-regulated gene expression.

Increased antibiotic susceptibility in the setting of pknA and pknB depletion

Table 1. Lowest inhibitory concentrations of antibiotics in wild type and kinase depletion strains (μg/ml).

Differential phosphorylation in kinase depletion strains and candidate substrate identification

Fig 7. Phosphoproteomic analysis of kinase depletion strains.

PknA and PknB target pathways required for growth and virulence

Discussion

Materials and methods

Strains, media, reagents, plasmids and primers

Generation of conditional depletion strains in M. tuberculosis

Analysis of inducible gene expression by fluorescence-activated cell sorting (FACS) assays

Assessing gene essentiality for replication and viability

RNA and total protein extraction

RT-qPCR

Western blotting

Acid-fast staining

Microscopy

Microplate Alamar Blue assay (MABA) for antibiotic susceptibility testing

Protein sample preparation, mass spectrometry and phosphopeptide identification

Differential abundance testing of phosphopeptides between M. tuberculosis strains

pLogo generation

Total lipid extraction, cell wall-bound mycolic acids (MA) isolation, and thin-layer chromatography (TLC)

13C labeling of M. tuberculosis mycolic acids and mass spectrometry analysis

Supporting information

Acknowledgments

Data Availability

Funding Statement

References

Associated Data

Supplementary Materials

Data Availability Statement

ACTIONS

PERMALINK

RESOURCES

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¹³C labeling of M. tuberculosis mycolic acids and mass spectrometry analysis