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. 2002 Jan 4;66(3):417–420. doi: 10.1002/jmv.2161

Human picornavirus and coronavirus RNA in nasopharynx of children without concurrent respiratory symptoms

Johanna Nokso‐Koivisto 1,2,✉, Teemu J Kinnari 2, Päivi Lindahl 3, Tapani Hovi 1, Anne Pitkäranta 2
PMCID: PMC7166414  PMID: 11793396

Abstract

The prevalence of human rhino‐, entero‐, and coronaviruses was investigated by RT‐PCR in nasopharyngeal aspirates from 107 children without concurrent respiratory symptoms. The children were admitted to the hospital for elective surgery. The parents filled a questionnaire about the occurrence of respiratory symptoms four weeks before and two weeks after the surgery. The rate of viral detection was 45% in children with related past or recent respiratory infection whereas 20% of the samples taken from children without any related past or recent respiratory infections were positive for picornavirus RNA, P = 0.008. Thirty‐one (29%) of the nasopharyngeal aspirates were positive for viral RNA, 18% for rhinovirus, and 11% for enterovirus RNA. Coronavirus RNA was not found in any of the children. Fifty‐five percent of the children with virus‐positive samples had an infection‐related diagnosis. In addition, 81% of the children with virus‐positive samples had had previously respiratory symptoms or there were concurrent respiratory symptoms in other family members. Only four of the 31 virus‐positive samples were from children without infection‐related diagnosis or recent past (or immediate future) respiratory symptoms. J. Med. Virol. 66:417‐420, 2002. © 2002 Wiley‐Liss, Inc.

Keywords: respiratory infection, PCR, rhinovirus, enterovirus

REFERENCES

  1. Bernstein JM. 1999. Waldeyer's ring and otitis media: the nasopharyngeal tonsil and otitis media. Int J Pediatr Otorhinolaryngol 49(Suppl 1): S127–132. [DOI] [PubMed] [Google Scholar]
  2. Blomqvist S, Skyttä A, Roivainen M, Hovi T. 1999. Rapid detection of human rhinovirus in nasopharyngeal aspirates by a microwell reverse trascription‐PCR‐hybridization assay. J Clin Microbiol 37: 2813–2816. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Faden H. 1998. Monthly prevalence of group A, B and C Streptococcus haemophilus influenzae types E and F and Pseudomonas aeruginosa nasopharyngeal colonization in the first two years of life. Pediatr Infect Dis J 17: 255–256. [DOI] [PubMed] [Google Scholar]
  4. Faden H, Duffy L, Wasielewski R, Wolf J, Krystofik D, Tung Y. 1997. Relationship between nasopharyngeal colonization and the development of otitis media in children. J Infect Dis 175: 1440–1445. [DOI] [PubMed] [Google Scholar]
  5. Halonen P, Rocha E, Hierholzer J, Holloway B, Hyypia T, Hurskainen P, Pallansch M. 1995. Detection of enteroviruses and rhinoviruses in clinical specimens by PCR and liquid‐phase hybridization. J Clin Microbiol 33: 648–653. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Heinonen P, Iitia A, Torresani T, Lovgren T. 1997. Simple triple‐label detection of seven cystic fibrosis mutations by time‐resolved fluorometry. Clin Chem 43: 1142–1150. [PubMed] [Google Scholar]
  7. Hendley J, Gwaltney JJ. 1988. Mechanisms of transmission of rhinovirus infections. Epidemiol Rev 10: 243–258. [PubMed] [Google Scholar]
  8. Horn M, Brain E, Gregg I, Inglis J, Yealland S, Taylor P. 1979. Respiratory viral infection and wheezy bronchitis in childhood. Thorax 34: 23–28. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hudgel DW, Jr LL, Selner JC, McIntosh K. 1979. Viral and bacterial infections in adults with chronic asthma. Am Rev Respir Dis 120: 393–397. [DOI] [PubMed] [Google Scholar]
  10. Ieven M, Goossens H. 1997. Relevance of nucleic acid amplification techniques for diagnosis of respiratory tract infections in the clinical laboratory. Clin Microbiol Rev 10: 242–256. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Jennings L, Barns G, Dawson K. 1987. The association of viruses with acute asthma. NZ Med J 12: 488–490. [PubMed] [Google Scholar]
  12. Johnston S, Sanderson G, Pattemore P, Smith S, Bardin P, Bruce C, Lambden P, Tyrrell D, Holgate S. 1993. Use of polymerase chain reaction for diagnosis of picornavirus infection in subjects with and without respiratory symptoms. J Clin Microbiol 31: 111–117. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Monto A. 1994. Studies of the community and family: acute respiratory illness and infection. Epidemiol Rev 16: 351–373. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Nokso‐Koivisto J, Pitkäranta A, Blomqvist S, Kilpi T, Hovi T. 2000. Respiratory coronavirus infections in children younger than two years of age. Pediatr Infect Dis J 19: 164–166. [DOI] [PubMed] [Google Scholar]
  15. Pitkäranta A, Arruda E, Malmberg H, Hayden FG. 1997. Detection of rhinovirus in sinus brushings of patients with acute community‐aquired sinusitis by reverse transcription‐PCR. J Clin Microbiol 35: 1791–1793. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Vesa S, Kleemola M, Blomqvist S, Takala A, Kilpi T, Hovi T. 2001. Epidemiology of documented viral respiratory infections and acute otitis media in a cohort of children followed from two to twenty‐four months of age. Pediatr Infect Dis J 20: 574–580. [DOI] [PubMed] [Google Scholar]

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