Skip to main content
NCBI home page
Wiley - PMC COVID-19 Collection logoLink to Wiley - PMC COVID-19 Collection
. 2005 Dec 6;9(1):43–49. doi: 10.1002/jmv.1890090107

Enzyme‐Linked lmmunosorbent assay: A rapid reproducible test for the measurement of rabies antibody

Karl G Nicholson 1,✉, Howard Prestage 1
PMCID: PMC7166757  PMID: 7038045

Abstract

An enzyme‐linked immunosorbent assay (ELISA) was developed for detecting IgG antibodies to the Pitman Moore strain of rabies virus in sera from subjects immunised with HDCS vaccine. End‐point titres of antibody were determined using a pocket calculator preprogrammed to analyse absorbence values of test sera and negative controls. The assay was highly reproducible, and very close agreement was achieved when the results were compared with those of the mouse neutralization test. Rabies enzyme‐linked immunosorbent assay is easy to perform, the results are obtained within 48 hours, and because it employs inactivated virus, the test can be used safely without special containment facilities.

Keywords: rabies virus, ELISA, end‐point titres, preprogrammed pocket calculator

References

  1. Atanasiu P (1973): Quantitative assay and potency test of antirabies serum and immunoglobulin. WHO [PubMed]
  2. Monograph Series 23: 314–318. [PubMed] [Google Scholar]
  3. Antanasiu P, Savy V, Perrin P (1977): Epreuve immunoenzymatique pour la détection rapide des anticorps antirabiques. Ann Microbiol (Inst Pasteur) 128 A: 489–498. [PubMed] [Google Scholar]
  4. Barth R, Gross‐Albenhausen E, Jaeger O, Milcke L (1981): The antibody binding test, a useful method for quantitative determination of inactivated rabies virus antigen. J Biol Stand 9: 81–89. [DOI] [PubMed] [Google Scholar]
  5. Fitzgerald EA, Baer G, Cabasso VJ, Vallancourt RJ (1975): A collaborative study on the potency testing of antirabies globulin. J Biol Stand 3: 273–278. [DOI] [PubMed] [Google Scholar]
  6. Harboe A, Borthne B, Berg K (1961): Antibody against normal egg material resulting from influenza vaccination. Acta Pathol 53: 95–103. [DOI] [PubMed] [Google Scholar]
  7. Hilfenhaus J, Köhler R, Barth R, Majer M, Mauler R (1976): Large‐scale purification of animal viruses in the R K‐model zonal ultracentrifuge. 1. Rabies virus. J Biol Stand 4: 263–271. [DOI] [PubMed] [Google Scholar]
  8. Kraaijeveld CA, Reed SE, Macnaughton MR (1980): Enzyme linked immunosorbent assay for detection of antibody in volunteers experimentally infected with human coronavirus 229 E group viruses. J Clin Microbiol 12: 493–497. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kuwert EK, Thraenhart O, Marcus I, Werner J, Atanasiu P, Bahmanyar M, Bögel K, Cox JH, Schneider LG, Turner G, Wiktor TJ (1978): Comparative study on antibody determination by different methods in sera of persons vaccinated with HDCS rabies vaccine. Joint WHO/IABS Symposium on the standardization of rabies vaccines for human use produced in tissue cultures (Rabies III) Marburg/Lahn, 1977.
  10. Lorenz RJ, Bögel K (1973): Methods of Calculation: The Spearman‐Kórber method. EHO Monograph Series 23: 321–329. [PubMed] [Google Scholar]
  11. Nicholson KG, Turner GS, Aoki FY (1978): Immunisation with a human diploid cell strain of rabies virus vaccine: Two year results. J Infect Dis 137: 783–788. [DOI] [PubMed] [Google Scholar]
  12. Nicholson KG, Harrison P, Turner GS (1979): Rabies neutralizing antibody determination by the interference inhibition test (IIT) and the mouse neutralization test (MNT(. J Biol Stand 7: 251–259. [DOI] [PubMed] [Google Scholar]
  13. Sedwick WD, Wiktor TJ (1967): Reproducible plaquing system for rabies, lymphocytic choriomeningitis and other riboneucleic acid viruses in BHK‐21/135 agarose suspensions. J Virol 1: 1224–1226. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sever JT, Madden DL (ed) (1977): Enzyme‐linked immunosorbent assay (ELISA) for infectious agents. J Infect Dis 136: S257–S340. [DOI] [PubMed] [Google Scholar]
  15. Snedecor GW, Cochran WG (1967): Regression In “Statistical Methods,” ed 6 The Iowa State University Press, ch 6, pp 135–171. [Google Scholar]
  16. Smith JS, Yager PA, Baer GM (1973): A rapid reproducible test for determining rabies neutralizing antibody. Bull WHO 48: 535–541. [PMC free article] [PubMed] [Google Scholar]
  17. Thraenhart O, Kuwert EK (1977): Enzyme immunoassay for demonstration of rabies virus antibodies after immunisation (letter) Lancet 2: 399–400. [DOI] [PubMed] [Google Scholar]
  18. Turner GS (1978): Immunoglobulin (IgG) and (IgM) antibody responses to rabies vaccine. J Gen Virol: 40: 595–604. [DOI] [PubMed] [Google Scholar]
  19. Wiktor TJ, Gyorgy E, Schlumberger HD, Sokol F, Koprowski H (1973): Antigenic properties of rabies virus components. J Immunol 110: 269–276. [PubMed] [Google Scholar]

Articles from Journal of Medical Virology are provided here courtesy of Wiley

RESOURCES