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. 2007 Jun 27;79(8):1238–1243. doi: 10.1002/jmv.20926

Detection of viruses identified recently in children with acute wheezing

Ju‐Young Chung 1,✉, Tae Hee Han 2, Sang Woo Kim 1, Chang Keun Kim 1, Eung‐Soo Hwang 3
PMCID: PMC7166823  PMID: 17597481

Abstract

The etiologic role of recently identified respiratory viruses for acute wheezing in children is not yet clear. The purpose of this study was to investigate the prevalence of recently identified viruses, including human metapneumovirus (hMPV), human bocavirus (hBoV), human coronavirus NL63 (hCoV‐NL63), and human coronavirus HKU1 (hCoV‐HKU1) in children with acute wheezing. Viral etiology was identified in 231 children hospitalized with acute wheezing, aged from 1 month to 5 years. Viral antigens for common respiratory viruses were detected by IFA or multiplex PCR. RT‐PCR was used to detect respiratory rhinoviruses, hCoV‐NL63, hCoV‐HKU1, and hMPV. PCR assays for hBoV DNA were performed using the primer sets for noncapsid protein (NP1) and nonstructural protein (NS1) genes. Viruses were found in 61.5% (142/231) of the study population and a single virus was detected in 45.5% (105/231) of the study population. Rhinovirus (33.3%), human respiratory syncytial virus (hRSV; 13.8%), and hBoV (13.8%) were the most frequently detected viruses. hMPV and hCoV‐NL63 were detected in 7.8% and 1.3% of wheezing children, respectively. HCoV‐HKU1 was not detected. In 16.0% of the study population, more than one virus was detected. In children with acute wheezing, rhinovirus, hRSV, and hBoV were most frequently detected. Further studies including healthy control subjects are needed to define the clinical significance of hBoV in acute wheezing. J. Med. Virol. 79: 1238–1243, 2007. © 2007 Wiley‐Liss, Inc.

Keywords: wheezing, human bocavirus, rhinovirus, RT‐PCR

REFERENCES

  1. Allander T, Tammi MT, Eriksson M, Bjerkner A, Tiveljung‐Lindell A, Andersson B. 2005. Cloning of a human parvovirus by molecular screening of respiratory tract samples. Proc Natl Acad Sci USA 101: 12891–12896. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Andreoletti L, Lesay M, Deschildre A, Lambert V, Dewilde A, Wattre P. 2000. Differential detection of rhinoviruses and enteroviruses RNA sequences associated with classical immunofluorescence assay detection of respiratory virus antigens in nasopharyngeal swabs from infants with bronchiolitis. J Med Virol 61: 341–346. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Arden KE, Nissen MD, Sloots TP, Mackay IM. 2005. New human coronavirus, HCoV NL63, associated with severe lower respiratory tract disease in Australia. J Med Virol 75: 455–462. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Arden KE, McErlean P, Nissen MD, Sloots TP, Mackay IM. 2006. Frequent detection of human rhinoviruses, paramyxoviruses, coronaviruses, and bocavirus during acute respiratory tract infections. J Med Virol 78: 1232–1240. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Arnold JC, Singh KK, Spector AA, Sawyer MH. 2006. Human bocavirus: Prevalence and clinical spectrum at a children's hospital. Clin Infect Dis 43: 283–288. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Bellau‐Pujol S, Vabret A, Legrand L, Dina J, Gouarin S, Petitjean‐Lecherbonnier J, Pozzetto B, Ginevra C, Freymuth F. 2005. Development of three multiplex RT‐PCR assays for the detection of 12 respiratory RNA viruses. J Virol Methods 126: 53–63. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Bosis S, Esposito S, Niesters HG, Crovari P, Osterhaus AD, Principi N. 2005. Impact of human metapneumovirus in childhood; comparison with respiratory syncytial virus and influenza viruses. J Med Virol 75: 101–104. [DOI] [PubMed] [Google Scholar]
  8. Chiu SS, Lau YL, Chan KH, Wong WH, Peiris JS. 2002. Influenza related hospitalizations among children in Hong Kong. N Engl J Med 347: 2097–2103. [DOI] [PubMed] [Google Scholar]
  9. Chung JY, Han TH, Kim CG, Kim SW. 2006. Bocavirus infection in hospitalized children, South Korea. Emerg Infect Dis 12: 1254–1256. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Cunningham AF, Johnston SL, Julious SA, Lampe FC, Ward ME. 1998. Chronic Chlamydia pneumoniae infection and asthma exacerbations in children. Eur Respir J 11: 345–349. [DOI] [PubMed] [Google Scholar]
  11. Freymuth F, Vabret A, Brouard J, Toutain F, Verdon R, Petitjean J, Duhamel JF, Guillois B. 1999. Detection of viral, Chlamydia pneumoniae and Mycoplasma pnumoniae infections in exacerbations of asthma in children. J Clin Virol 13: 131–139. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gama RE, Horsnell PR, Hughes PJ, North C, Bruce CB, al‐Nakib W, Stanway G. 1989. Amplification of rhinovirus specific nucleic acids from clinical samples using polymerase chain reaction. J Med Virol 28: 73–77. [DOI] [PubMed] [Google Scholar]
  13. Han TH, Chung JY, Kim SW, Hwang ES. 2007. Human coronavirus‐NL63 infections in Korean children. 2004–2006. J Clin Virol 38: 27–31. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Heymann PW, Carper HT, Murphy DD, Plat‐Mills TA, Patrie J, McLaughlin AP, Erwin EA, Shaker MS, Hellems M, Peerzada J, Hayden FG, Haltey TK, Chamberlain R. 2004. Viral infections in relation to age, atopy, and season of admission among children hospitalized for wheezing. J Allergy Clin Immunol 114: 239–247. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Heymann PW, Platts‐Mills TA, Johnston SL. 2005. Role of viral infections, atopy, antiviral immunity in the etiology of wheezing exacerbation among children and young adults. Pediatr Infect Dis J 24: S217–S222. [DOI] [PubMed] [Google Scholar]
  16. Jacque J, Bouscambert‐Duchamp M, Moret H, Carquin J, Brodard V, Lina B, Motte J, Andreoletti L. 2006. Association of respiratory picornaviruses with acute bronchiolitis in French infants. J Clin Virol 35: 463–466. [DOI] [PubMed] [Google Scholar]
  17. Jartti T, Lehtinen P, Vuorinen T, Osterback R, van den Hoogen B, Osterhaus AD, Ruuskanen O. 2004. Respiratory picornaviruses and respiratory syncytial virus as causative agents of acute expiratory wheezing in children. Emerg Infect Dis 10: 1095–1101. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Johnston SL, Sanderson G, Pattemore PK, Smith S, Bardin PG, Bruce CB, Lambden PR, Tyrell DA, Holgate ST. 1993. Use of polymerase chain reaction for diagnosis of picornavirus infection in subjects with and without respiratory symptoms. J Clin Microbiol 31: 111–117. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Johnston SL, Pattermore PK, Sanderson G, Smith S, Lampe F, Josephs L, Symington P, O'Toole S, Myint SH, Tyrell DA. 1995. Community study of the role of viral infections in exacerbations in 9–11 years old children. BMJ 310: 1225–1229. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Johnston NW, Johnston SL, Duncan JM, Greene JM, Kebadze T, Keith PK, Roy M, Waserman S, Sears MR. 2005. The September epidemic of asthma exacerbation in children: A search for etiology. J Allergy Clin Immunol 115: 132–138. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Kesebir D, Vazquez M, Weibel C, Shapiro ED, Ferguson D, Landry ML, Kahn JS. 2006. Human bocavirus infection in young children in the United States: Molecular epidemiological profile and clinical characteristics of a newly emerging respiratory virus. J Infect Dis 194: 1276–1282. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Khetsuriani N, Kazerouni NN, Erdman DD, Lu X, Redd SC, Anderson LJ, Teaque WG. 2007. Prevalence of viral respiratory tract infections in children with asthma. J Allergy Clin Immunol 119: 314–321. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Lemanske RF, Jr. , Jackson DJ, Gangnon RE, Evans MD, Li Z, Shult PA, et al. 2005. Rhinovirus illness during infancy predicts subsequent childhood wheezing. J Allergy Clin Immunol 116: 571–577. [DOI] [PubMed] [Google Scholar]
  24. Ma X, Endo R, Ishiquro N, Ebihara T, Ishiko H, Ariga T, Kikuta H. 2006. Detection of human bocavirus in Japanese children with lower respiratory tract infections. J Clin Microbiol 44: 1132–1134. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Mertsola J, Ziegler T, Ruuskanen O, Vanto T, Koivikko A, Halonen P. 1991. Recurrent wheezy bronchitis and viral respiratory infections. Arch Dis Child 66: 124–129. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Nagayama Y, Tsubaki T, Nakayama S, Swada K, Taguchi K, Tateno N, Toba T. 2006. Gender analysis in acute bronchiolitis due to respiratory syncytial virus. Pediatr Allergy Immunol 17: 29–36. [DOI] [PubMed] [Google Scholar]
  27. Papadopoulos NG, Moustaki M, Tsolia M, Bossios A, Astra E, Prezerakou A, Gourgiotis D, Kafetzis D. 2002. Association of rhinovirus infection with increased disease severity in acute bronchiolitis. Am J Respir Crit Care Med 165: 1285–1289. [DOI] [PubMed] [Google Scholar]
  28. Rakes GP, Arruda E, Ingram GM, Hoover GE, Zambrano JC, Hayden FG, Platts‐Mills TA, Heymann P. 1999. Rhinovirus and respiratory syncytial virus in wheezing children requiring emergency care. IgE and eosinophil analyses. Am J Respir Crit Care Med 159: 785–790. [DOI] [PubMed] [Google Scholar]
  29. Rawlinson WD, Waliuzzaman Z, Carter JW, Belessis YC, Gilbert KM, Morton JR. 2003. Asthma exacerbation in children associated with rhinovirus but not human metapneumovirus infection. J Infect Dis 187: 1314–1318. [DOI] [PubMed] [Google Scholar]
  30. Sigurs N, Bjarnason R, Sigurbergsson F, Kjellman B. 2000. Respiratory syncytial virus bronchiolitis in infancy is an important risk factor for asthma and allergy at age 7. Am J Respir Crit Care Med 161: 1501–1507. [DOI] [PubMed] [Google Scholar]
  31. Sloots TP, McErlean P, Speicher DJ, Arden KE, Nissen MD, MacKay IM. 2006. Evidence of human coronavirus HKU‐1 and human bocavirus in Australian children. J Clin Virol 35: 99–102. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Thumerelle C, Deschildre A, Bouquillon C, Santos C, Sardet A, Scalbert M, Delbecque I, Debray P, Dewilde A, Turck D, Leclerc F. 2003. Role of viruses and atypical bacteria in exacerbations of asthma in hospitalized children: A prospective study in the Nord‐Pas de Calais region (France). Pediatr Pulmonol 35: 75–82. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Vabret A, Dina J, Gouarin S, Petitjean J, Corbet S, Freymuth F. 2006. Detection of the new human coronavirus HKU‐1. Clin Infect Dis 42: 634–639. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. van den Hoogen BG, de Jong JC, Groen J, Groen J, Kuiken T, de Groot R, Fouchier RA, Osterhaus AD. 2001. A newly discovered human pneumovirus isolated from young children with respiratory tract disease. Nat Med 7: 719–724. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. van den Hoogen BG, Herfst S, Sprong L, Cane PA, Forleo‐Neto E, de Swart RL, Osterhaus AD, Fouchier RA. 2004. Antigenic and genetic variability of human metapneumoviruses. Emerg Infect Dis 10: 658–666. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. van der Hoek L, Sure K, Ihorst G, Stang A, Pyrc K, Jebbink MF, Peterson G, Forster J, Berkhout B, Uberla K. 2005. Croup is associated with the novel coronavirus NL63. Plos Med 2: e240. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Weissbrich BB, Neske F, Schubert J, Tollmann F, Blath K, Blessing K, Kreth W. 2006. Frequent detection of bocavirus DNA in German children with respiratory tract infections. BMC Infect Dis 6: 109. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Williams JV, Tollefson SJ, Heymann PW, Carper HT, Patrie J, Crowe JE. 2005. Human metapneumovirus infection in children hospitalized for wheezing. J Allergy Clin Immunol 115: 1311–1312. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. Woo PC, Lau SK, Tsoi HW, Huang Y, Poon RW, Chu CM, Lee RA, Luk WK, Wong GK, Wong BH, Cheng WC, Tang BS, Wu AK, Yung RW, Chen H, Guan Y, Chan KH, Yuen KY. 2005. Clinical and molecular epidemiological features of coronavirus HKU1‐associated community‐acquired pneumonia. J Infect Dis 192: 1898–1907. [DOI] [PMC free article] [PubMed] [Google Scholar]

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