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. 2004 Oct 13;74(4):517–520. doi: 10.1002/jmv.20207

Evaluation of a peptide‐based enzyme immunoassay for anti‐SARS coronavirus IgG antibody

Paul KS Chan 1,2,✉, WK To 3, Esther YM Liu 2, TK Ng 3, John S Tam 1,2, Joseph JY Sung 1, Jean‐Michel Lacroix 4, Michel Houde 4
PMCID: PMC7166921  PMID: 15484283

Abstract

High throughput assays for anti‐SARS‐CoV IgG antibody detection are need for large‐scale epidemiologic studies. The performance of a microplate enzyme immunoassay, DETECT‐SARS™, was evaluated for the detection of anti‐SARS‐CoV IgG antibody. This assay is based on synthetic peptides derived from the nucleocapsid and spike proteins. The results showed that the assay provided a high degree of sensitivity (95.9%) for convalescent serum samples. The level of specificity was close to 90%, and did not show significant variation among different control groups. The high degree of sensitivity together with the high‐throughput nature makes it advantageous as a screening assay for studies where handling of a large number of specimens is required. J. Med. Virol. 74:517–520, 2004. © 2004 Wiley‐Liss, Inc.

Keywords: coronavirus, enzyme immunoassay, IgG, SARS, serology

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