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. 2005 Dec 7;31(2):165–172. doi: 10.1002/jmv.1890310216

Characterization of a nucleic acid probe for the diagnosis of human coronavirus 229E infections

S Myint 1,3,✉, D Harmsen 2, T Raabe 2, S G Siddell 2
PMCID: PMC7167089  PMID: 2167350

Abstract

A cDNA copy of the HCV229E nucleocapsid protein gene was isolated and characterized. Sequence analysis predicts a nucleocapsid polypeptide of 389 amino acids with a molecular weight (mol. wt.) of 43,450. Single strand RNA probes derived from the cDNA copy hybridize specifically to HCV229E RNA and approximately 50 pg of intracellular viral RNA can be readily detected. The application of nucleic acid hybridization as a routine procedure for the diagnosis of HCV229E infection is discussed.

Keywords: N gene sequence, hybridization analysis, coronavirus

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