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. 2013 Nov 14;25(10):1019–1024. doi: 10.1111/j.1348-0421.1981.tb00108.x

Comparison of Three Electron Microscopy Techniques for the Detection of Human Rotaviruses

Pierre Trépanier 1,2, Robert Alain 1,2, Valeriu Micusan 1,2, Bernadette McLaughlin 1,2, Laurent Berthiaume 1,2
PMCID: PMC7168361  PMID: 6273695

Abstract

Rotavirus detection by direct electron microscopy was compared with direct and indirect immune electron microscopy techniques. The latter two approaches permitted the enumeration of 25 and 103 times more rotaviruses respectively, than direct electron microscopy. Also, 70% and 90% of the virus particles were aggregated by direct and indirect immune electron microscopy techniques respectively, thus facilitating their detection.

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