Abstract
Complete nucleotide sequences were determined by cDNA cloning of peplomer (S), integral membrane (M) and nucleocapsid (N) genes of feline infectious peritonitis virus (FIPV) type I strain KU‐2, UCD1 and Black, and feline enteric coronavirus (FECV) type II strain 79–1683. Only M and N genes were analyzed in strain KU‐2 and strain 79–1683, which still had unknown nucleotide sequences. Deduced amino acid sequences of S, M and N proteins were compared in a total of 7 strains of coronaviruses, which included FIPV type II strain 79–1146, canine coronavirus (CCV) strain Insavc‐1 and transmissible gastroenteritis virus of swine (TGEV) strain Purdue. Comparison of deduced amino acid sequences of M and N proteins revealed that both M and N proteins had an identity of at least 90% between FIPV type I and type II. The phylogenetic tree of the M and N protein‐deduced amino acid sequences showed that FIPV type I and type II form a group with FECV type II, and that these viruses were evolutionarily distant from CCV and TGEV. On the other hand, when the S protein‐deduced amino acid sequences was compared, identity of only about 45% was found between FIPV type I and type II. The phylogenetic tree of the S protein‐deduced amino acid sequences indicated that three strains of FIPV type I form a group, and that it is a very long distance from the FIPV type II, FECV type II, CCV and TGEV groups.
Keywords: feline infectious peritonitis virus, feline enteric coronavirus, amino acid sequence
Abbreviations
- CCV
canine coronavirus
- fcwf 4
Felis catus whole fetus cells
- FECV
feline enteric coronavirus
- FIPV
feline infectious peritonitis virus
- kb
kilobases
- kDa
kilodaltons
- M protein
integral membrane glycoprotein
- M gene
integral membrane gene
- N protein
nucleocapsid protein
- N gene
nucleocapsid gene
- ORF
open reading frame
- RT
reverse transcription
- S protein
peplomer glycoprotein
- S gene
peplomer gene
- TGEV
transmissible gastroenteritis virus of swine
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