Skip to main content
NCBI home page
Wiley - PMC COVID-19 Collection logoLink to Wiley - PMC COVID-19 Collection
. 2013 Nov 14;24(9):825–834. doi: 10.1111/j.1348-0421.1980.tb02887.x

Hydrocephalus in Suckling Rats Infected Intracerebrally with Mouse Hepatitis Virus, MHV‐A59

Norio Hirano 1,2, Naoaki Goto 1,2, Tetsuo Ogawa 1,2, Katsuhiko Ono 1,2, Toshiaki Murakami 1,2, Kosaku Fujiwara 1,2
PMCID: PMC7168494  PMID: 6261095

Abstract

After intracerebral inoculation of mouse hepatitis virus, MHV‐A59 strain, into 3‐ to 5‐day‐old Wistar rats, some survivors at 14 days postinoculation (p.i.) were found to lack the cerebral cortex and to have an accumulation of a considerable amount of cerebrospinal fluid. The virus titer in the brain increased exponentially after inoculation, reaching a maximum 4 to 6 days p.i. when immunofluorescence revealed virus‐specific antigen within neurons in the cerebral cortex. A small amount of infectious virus was also detectable 14 days p.i. when the cerebral anomaly was evident. This brain malformation causing hydrocephalus was due to cerebral damage by viral infection.

REFERENCES

  • 1. Bailey, O.T. , Pappenheimer, A.M. , Cheever, F.S. , and Daniels, J.B. 1949. A murine virus (JHM) causing disseminated encephalomyelitis with extensive destruction of myelin. II. Pathology. J. Exp. Med. 90: 195–212. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 2. Bhatt, P.N. , and Jacoby, R.O. 1977. Experimental infection of adult axenic rats with Parker's rat coronavirus. Arch. Virol. 54: 345–352. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 3. Bhatt, P.N. , Jacoby, R.O. , and Jonas, A.M. 1977. Respiratory infection in mice with sialodacryoadenitis virus, a Coronavirus of rats. Infect. Immun. 18: 823–827. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 4. Bhatt, P.N. , Percy, D.H. , and Jonas, A.M. 1972. Characterization of the virus of sialodacryoadenitis of rats: A member of the coronavirus group. J. Infect. Dis. 126: 123–130. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 5. Broderson, J.R. , Murphy, F.A. , and Hierholzer, J.C. 1976. Lethal enteritis in infant mice caused by mouse hepatitis virus. Lab. Anim. Sci. 26: 824–825. [PubMed] [Google Scholar]
  • 6. Cheever, F.S. , Daniels, J.B. , Pappenheimer, A.M. , and Bailey, O.T. 1949. A murine virus (JHM) causing disseminated encephalomyelitis with extensive destruction of myelin. I. Isolation and biological properties of the virus. J. Exp. Med. 90: 181–194. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 7. Chew‐Lim, M. , and Webb, H.E. 1976. Mouse hydrocephalus and encephalitis following intracerebral infection with Newcastle disease vaccine viruses. Br. J. Exp. Pathol. 57: 472–480. [PMC free article] [PubMed] [Google Scholar]
  • 8. Dick, G.W.A. , Niven, J.F.S. , and Gledhill, A.W. 1956. A virus related to the causing hepatitis in mice (MHV). Br. J. Exp. Pathol. 37: 90–97. [PMC free article] [PubMed] [Google Scholar]
  • 9. Fujiwara, K. 1969. Problems in checking inapparent infection in laboratory mouse colonies: Attempts at serological checking by anamnestic response. In Defining of the laboratory animals. Natl. Acad. Sci. Wash. D.C. 77–92. [Google Scholar]
  • 10. Fujiwara, K. , Takenaka, S. , and Shumiya, S. 1976. Carrier state of antibody and virus in a mouse breeding colony persistently infected with Sendai and mouse hepatitis viruses. Lab. Anim. Sci. 26: 153–159. [PubMed] [Google Scholar]
  • 11. Fujiwara, K. , Tanishima, Y. , and Tanaka, M. 1978. Seromonitoring of laboratory mouse and rat colonies for common murine pathogens. Exp. Anim. 28: 297–306. [DOI] [PubMed] [Google Scholar]
  • 12. Gledhill, A.W. , and Andrewes, C.H. 1951. A hepatitis virus of mice. Br. J. Exp. Pathol. 32: 559–568. [PMC free article] [PubMed] [Google Scholar]
  • 13. Goto, N. , Hirano, N. , Aiuchi, M. , Hayashi, T. , and Fujiwara K. 1977. Nasoencephalopathy of mice infected intracerebrally with a mouse hepatitis virus, JHM strain. Japan. J. Exp. Med. 47: 59–70. [PubMed] [Google Scholar]
  • 14. Hirano, N. , Fujiwara, K. , Hino, S. , and Matumoto, M. 1974. Replication and plaque formation of mouse hepatitis virus (MHV‐2) in mouse cell line DBT culture. Arch. Gesamte Virusforsch. 44: 298–302. [DOI] [PubMed] [Google Scholar]
  • 15. Hirano, N. , Fujiwara, K. , and Matumoto, M. 1976. Mouse hepatitis virus (MHV‐2). Plaque assay and propagation in mouse cell line DBT cells. Japan. J. Microbiol. 20: 219–225. [PubMed] [Google Scholar]
  • 16. Hirano, N. , Murakami, T. , Fujiwara, K. , and Matumoto, M. 1978. Utility of mouse cell line DBT for propagation and assay of mouse hepatitis virus. Japan. J. Exp. Med. 48: 71–75. [PubMed] [Google Scholar]
  • 17. Hirano, N. , Takenaka, S. , and Fujiwara, K. 1975. Pathogenicity of mouse hepatitis virus for mice depending upon host age and route of infection. Japan. J. Exp. Med. 45: 285–292. [PubMed] [Google Scholar]
  • 18. Hirano, N. , Tamura, T. , Taguchi, F. , Ueda, K. , and Fujiwara, K. , 1975. Isolation of low‐virulent mouse hepatitis virus from nude mice with wasting syndrome and hepatitis. Japan. J. Exd. Med. 45: 492–496. [PubMed] [Google Scholar]
  • 19. Jacoby, R.O. , Bhatt, P.N. , and Jonas, A.M. 1975. Pathogenesis of sialodacryoadenitis in gnoto‐biotic rats. Vet. Pathol. 12: 169–209. [DOI] [PubMed] [Google Scholar]
  • 20. Johnson, R.T. , and Johnson, K.P. 1969. Hydrocephalus as a sequence of experimental myxo‐virus infections. Exp. Mol. Pathol. 10: 68–80. [DOI] [PubMed] [Google Scholar]
  • 21. Johnson, R.T. , Johnson, K.P. , and Edomonds, C.J. 1967. Virus‐induced hydrocephalus: Development of aqueductal stenosis in hamsters after mumps infection. Science 157: 1066–1067. [DOI] [PubMed] [Google Scholar]
  • 22. Kilham, L. , and Mongolis, G. 1969. Hydrocephalus in hamsters, ferrets, rats and mice following intracerebral inoculation with reovirus type 1. I. Virological studies. Lab. Invest. 21: 183–188. [PubMed] [Google Scholar]
  • 23. Lampart, P.W. , Sims, J.K. , and Knieaeff, A.J. 1975. Mechanism of demyelination in JHMV virus encephalomyelitis. Electron microscopic studies. Acta Neuropathol. 24: 76–85. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 24. London, W.T. , Fuccillo, D.A. , Sever, J.L. , and Kento, S.G. 1975. Influenza virus as a teratogen in rhesus monkeys. Nature 255: 483–484. [DOI] [PubMed] [Google Scholar]
  • 25. Manaker, R.A. , Piczak, C.V. , Miller, A.A. , and Stanton, M.F. 1961. A hepatitis virus complicating studies with mouse leukemia. J. Natl. Cancer Inst. 27: 29–45. [PubMed] [Google Scholar]
  • 26. McIntosh, K. 1974. Coronaviruses. A comparative review. Curr. Top. Microbiol. Immunol. 63: 85–129. [Google Scholar]
  • 27. Mims, C.A. , Murphy, F.A. , Taylor, W.P. , and Marshall, I.D. 1973. Pathogenesis of Ross River virus infection in mice. I. Ependymal infection, cortical thinning and hydrocephalus. J. Infect. Dis. 127: 121–128. [DOI] [PubMed] [Google Scholar]
  • 28. Nagashima, K. , Wege, H. , Meyrman, R. , and ter Meulen, V. 1978. Corona virus induced subacute demyelinating encephalomyelitis in rats. A morphological analysis. Acta Neuropathol. 44: 63–70. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 29. Nelson, J.B. 1952. Acute hepatitis associated with mouse leukemia. I. Pathological features and transmission of the disease. J. Exp. Med. 96: 293–303. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 30. Norrby, E. , and Kristensson, K. 1978. Subacute encephalitis and hydrocephalus in hamsters caused by measles virus from persistently infected cell cultures. J. Med. Virol. 2: 305–317. [DOI] [PubMed] [Google Scholar]
  • 31. Parker, J.C. , Cross, S.S. , and Rowe, W.P. 1970. Rat coronavirus (RCV): A prevalent, naturally occurring pneumotropic virus of rats. Arch. Gesamte Virusforsch. 31: 293–302. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 32. Parker, J.C. , Tennet, R.W. , Ward, T.C. , and Rowe, W.P. 1965. Virus studies with germfree mice. I. Preparation of serologic diagnostic reagents and survey of germfree and monocontaminated mice for indigenous murine viruses. J. Natl. Cancer Inst. 34: 371–380. [PubMed] [Google Scholar]
  • 33. Powell, H.C. , and Lampart, P.W. 1975. Oligodendrocytes and their myelinplasma membrane connection in JHM mouse hepatitis virus encephalitis. Lab. Invest. 33: 440–445. [PubMed] [Google Scholar]
  • 34. Rowe, W.P. , Hartley, J.W. , and Gapps, W.I. 1963. Mouse hepatitis virus infection as a highly contagious prevalent, enteric infection of mice. Proc. Soc. Biol. Med. 112: 161–165. [DOI] [PubMed] [Google Scholar]
  • 35. Tyrrell, D.A.J. , Almeida, J.D. , Cunningham, C.H. , Dowdle, W.R. , Hofstad, M.S. , McIntosh, K. , Tajima, M. , Zakstelskaya, L.Y. , Esterday, B.C. , Kapikian, A. , Bingham, R.W. 1975. Co‐ronaviridae. Intervirology 5: 76–82. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 36. Weiner, L.P. 1973. Pathogenesis of demyelination induced by mouse hepatitis virus (JHM strain). Arch. Neurol. 28: 293–303. [DOI] [PubMed] [Google Scholar]

Articles from Microbiology and Immunology are provided here courtesy of Wiley

RESOURCES